MrBayes: Bayesian Inference of Phylogeny
Dentre os softwares existentes para se fazer análises de inferência Bayesiana, um dos mais utilizados é o MrBayes 3.2 (Ronquist et al., 2012), então vamos analisar a nossa matriz nesse programa. Ele usa arquivos do tipo nexus e não possui interface gráfica. A primeira coisa que devemos fazer é abrir o arquivo hylodidae.nex em um editor de texto (bloco de notas, notepad, etc). Ele será mais ou menos assim:
#NEXUS
BEGIN DATA;
DIMENSIONS NTAX=24 NCHAR=3187;
FORMAT DATATYPE=DNA GAP=- MISSING=? ;
MATRIX
Limnomedusa_macroglossa ---TGGTCCTAGCCTTGAAGTCAGTTTTTACTCAATATATACATGCAAGTCTCTGCACCCCTGTGAGAACGCCCTTAAA-TCCCCGCTAGGGATAA-GGAGCCGGTATCAGGCGCAATAATCTGCCCAAGACACCTAGCTATGCCACACCCCCAAGGGAATTCAGCAGTAATTAACATTGAATATGAGCGCCAGCTTGATTCAGTTAGAGTAAACAGGGCCGGCTAATCTGGTGCCAGCCGCCGCGGTTACACCACGTGGCCCAAATTGACACTTTTCGGCGTAAAGCGTGATTAAAGTCATCCT--ATATTGAAGATTAATTTAAACTAAGCTGTGACACGCTTGTTATCAAGAAACTCAAACACGAAAGTTGCTTCAACCCTACCAACTTGAACTCACGACAGCTGGGGTACAAACTGGGATTAGATACCCCACTATGCCCAGCCGTAAACTTTAACCTACACCAC-CATCGCCTGGGAACTACGAGCAAAGCTTAAAACCCAAAGGACTTGACGGTACCCCACATCCACCTAGAGGAGCCTGTCCTGTAATCGATAACCCCCGTTAAACCTCACCATTTTTAGCTTATCAGCCTGTATACCTCCGTCGCCAGCTTACCACGTGAGCGAGATTTAGTGAGCTTAATGTCCATACGTCAGTACGTCAGGTCAAGGTGCAGCAAATAAAATGGGAAGAGATGGGCTACACTCTCTATAATAGAAGAAACGAAAGACTAATTATGAAA-CCTAGTCAGAAGGCGGATTTAGTAGTAAAGGGAAACAAGAGTGTTCACTTTAACTTGGCCCTGGGGTGTGTACACACCGCCCGTCACCCTCTTCA-AAGGTAATAGACAGTATTTAACAAGTTTTAG-CTTTTCAGAAGAGGCAAGTCGTAACATGGTAAGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCTTAA-CAAAAGCATCTCGCTTACACCGAGAAGTCATCTGTTAAAACCAGATTATTTTGAGCTAAAAATCTAG-CCCAACCTAAGCATATGAAAAAATAAAAATT-TTTTTACACTAATCAAAACATTCTTATTTCCTAGTAAAGGCGATTGAAAAGTCTAATTGGAGCTATATACAATGTACCGCAAGGGAAAGATGAAATAATAATGAAATAC--TCTCAAGCACAATTCAGTAGAGATTCAACCTCGTACCTTTTGCATCATGGTTTAACTAGTCTAATCAAGCAAAACGA-ACTTTAGTTTGACCTCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTATTGAGCCGACCCGTCTCTGTTGCAAAAGAGTGGGAAGACCTTCAAGTAGGGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTGCTCAAGAAAAGGATTTAAGTTCAGCCTAAAATTACCTCAA----TTTCACGAAATAAGGTCTTAATTTTAGAGTTATTCAAATAAGGTACAGCCTATTTGAAACAGGGTACAACCTAGATCAGCGGGTAATCTCTTTATA--TAATCTAACCAAGTAGGCCTAAAAGCAGCCACCTTTAA-AATAGCGTCACAGCTTAATTAAAT-GATTGATAAATCTCATTAAAATTTTAGAACCCCTACTCTATATTGAGCAATTCTATAGCC-CTATAGAACATATTATGTTAAAACTAGTAACAAGAAGAAAACCTTCTCTTTAATGTAAGTGTAAATCAGGTAGGATAAACCACTGATAATTAACATCCATGAGCTGAG-AGTATTAACCCATCAAGAAAACTATACTTCTTTATATGTTAACCTAACACAAGAGCATTTCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAATTTTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGATTAACAATAAGAGGTCCAGCCTGCCCAGTGAC-TCAGTTTAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTTTTTCCAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGGATTAAATTATAAGACGAGAAGACCCTATGGAGCTTTAAACAAAGTAACAATTGCTACTCTACTTTC-TAATTTCTGAAAACTAAC--TTATTTTAGCCTTATGA-TTACTAGTTTTAGGTTGGGGTGACCGCGGAGAATAATTTAACCTCCACATTGAATGAATT--CTATTCTAAGCTAAAAGCCACGACTTTAAGCATCAATAAATTGACATCTATTGATCCAATACTTTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTCAAGAGCTCCTATCGACAAGTGGGTTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT----------------------------------------------------------------------AACTCTCTATCTCATCTTCGGTGCCTGAGCCGGAATAGTGGGGACCGCCCTTAGTCTTCTTATCCGGGCTGAGCTAAGTCAGCCCGGCTCACTTCTAGGGGATGACCAAATTTACAATGTAATCGTAACCGCCCATGCCTTTGTTATAATCTTCTTCATGGTGATGCCAATTATAATTGGGGGTTTCGGAAACTGGCTCGTTCCTTTAATGATCGGGGCCCCAGACATGGCGTTCCCCCGAATGAACAATATAAGCTTCTGACTTCTCCCTCCCTCTTTCCTCTTACTTTTGGCCTCTGCAGGAGTGGAAGCCGGGGCCGGAACCGGGTGAACCGTTTACCCCCCTTTAGCAGGAAATCTAGCTCACGCCGGACCCTCCGTGGATTTAACTATTTTTTCTCTCCACCTAGCAGGTGTCTCTTCGATCCTGGGAGCAATTAACTTTATTACTACCATCCTAAACATAAAACCTCCCTCTATAACTCAATACCAAACGCCACTGTTTGTTTGGTCCGTATTAATTACTGCTGTTCTCCTCCTACTTTCACTCCCAGTCCTAGCCGCGGGCATTACTATACTTCTCACTGATCGTAACTTAAATACAACATTTTTTGACCCGGCAGGAGGGGGGGACCCAATCCTATACCAACACCTATTT-------
Crossodactylus_aeneus ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCGCCTGTTTATCAAAAACATCGCCTCTTGTTTATTTATAAGAGGTCCAGCCTGCCCAGTGAC-TCAATTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTTGTATGAATGGCATCACGAGGGTTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATAAACCTATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATAGCAATTGCTTATTT-TATAC-TATATCCCGATATTTAATACTTAATTTAGCATTATGAACTACAAATTTTAGGTTGGGGTGACCATGGAGCAAAAATCAACCTCCACATTGAATAAAACTTTACTTTTTAGCAAAAAGTTACCACTTTAAGCACCAACATATTGACATTAATTGACCCAATA--ATTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTAGGTTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAGTTCAGACCGGA????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Crossodactylus_bokermanni G------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAATAAGAGGTCTAGCCTGGCCAGTGAC-CTAGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTTTCTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAACATATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATAACAAGTGCTTCTTT-CTATCTCACATCCTGATGAATAATTTTTAATCTAGCATTTTAACTTATAGATTTTAGGTTGGGGTGACCATGGAGCAAAAACTAACCTCCATATTGAAAAACCT--CATTTTTAAGCAAAAAGTTACTACTTTAAGCATCAACATATTGACATTAATTGACCCAATA--ATTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTAGGTTTACGACCTCGATGTTGGATCAGGGTGTCCTAGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCT--------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Crossodactylus_caramaschii ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGAGGTCCTGCCTGCCCAGTGAC-CTAGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTCTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAATATATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATAACAAATGCTTATTT-TTTACTCTCATCCAGATGACTGACTATTATTTTAGCATTTTGACCTATAAATTTTAGGTTGGGGTGACCATGGAGCAAAAATTAACCTCCATATTGGAAAAACCT-TATTTTTAAGCAAAAAGTTACCACTTTAAGCATCAACACACTGACATTAATTGACCCAATA--GTTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTAGGCTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAAC--------------------------GTGATAATTACCCGATGATTTTTTTCTACCAACCACAAAGATATTGGAACCTTATACTTAGTATTTGGCGCATGAGCTGGAATGGTTGGGACCGCCCTCAGCCTGCTCATTCGAGCTGAATTAAGCCAGCCAGGCTCCCTGCTAGGCGATGACCAGATTTATAATGTTATTGTTACTGCACATGCTTTCGTAATAATTTTTTTTATAGTGATGCCGATTATAATCGGCGGATTCGGTAACTGACTAGTACCACTTATGATTGGGGCCCCTGATATAGCATTCCCCCGTATAAATAATATGAGCTTTTGACTCCTCCCCCCCTCATTTCTTCTTCTTTTGGCTTCCGCTGGGGTAGAGGCTGGGGCCGGAACCGGTTGAACTGTCTACCCCCCACTCGCCGGAAATATGGCCCATGCCGGACCATCCGTAGACTTAACAATTTTTTCTCTCCATCTTGCTGGAGTTTCCTCAATCTTAGGCGCAATTAACTTTATTACCACAATTCTAAATATAAAACCCCCCTCTGTTACCCAGTACCAGACCCCCTTATTTGTGTGGTCTGTCCTGATCACAGCTGTTCTTCTTCTCCTTTCCCTGCCTGTTTTAGCCGCCGGAATCACCATACTTCTCACGGACCGCAACCTAAATACTACATTTTTTGACCCGGCAGGTGGAGGAGACCCAA---------------------------
Crossodactylus_schmidti ---TGGTCCTAACCTTG-AATCAATTTTTACTGTACATACACATGCAAGTCTCCGGACCCCCGTGAAAACGCCCTTAAACTTCCTGCTAGGAACAAGGGAGCTGGTATCAGGCACAAC-TTCTGCCCAAAACACCTAGCTACGCCACACCCACAAGGGAATTCAGCAGTGATTAACATTGAATATTAGCGTCAGCTTGATTCAGTTAAAGTAAATAGAGCCGGCCAATCTGGTGCCAGCCGCCGCGGTTACACCACGTGGCTCAAATTGATTTTTACCGGCGTAAAGCGTGATTAAAGTCAACCCC-CTATTGAAGTCAAATTTAAACTAAGCCGTGACACGCTTGTTCTTTAGAAGCACAAAAACGAAAGTTACTTCATCTTAACCTACTTGAATACACGACAGCTTGAGCACAAACTGGGATTAGATACCCCACTATGTCCAGCCGTAAACTTTAAATTACTCCTTATACCGCCAGGGAACTACGAGCTAAGCTTAAAACCCAAAGGACTTGACGGTACCCCATGTCCCTCTAGAGGAGCCTGTCCTATAATCGATAACCCCCGTTTAACCTCACCATTTTTGGCTAATCAGTTTGTATACCTCCGTCGCCAGCTTACCCCGTGAGCGAATCTTAGTGAGCCTAACGCCCATACGCCAATACGTCAGGTCAAGGTGCAGCAAATAAAATGGAAAGAGATGGGCTACACTTTCTAATATAGAAAATACGAAAAACTACCTATGAAATCCTAGTTTGAAGGCGGATTTAGTAGTAAAAAGAAACCAGAGAGTTCTTTTTAACAAGGCCCTGGGGTGTGTACACACCGCCCGTCACCCTCTTCA-AAGCCAAATACCAGTTATTAACAAAATCTG--CTTAACAGAAGAGGCAAGTCGTAACATGGTAAGCATACCGGAAGGTGTGCTTGGAATCAAAGTATAGCTTAATCTAAAGCGCCTCGCTTACACCGAGCACATATTTGTTAAAATCAAATTACTTTGAGCTGAAAATCTAG-CCCCACATCTTCTTATGATCAAC-TTACACC-TAACTTTTTCCACTAAAACATTTTATTATTTAAGTAAAGGCGATCGAAAAAT-TAACAAGAGCGATAAATATTGTACCGTAAGGGAAAGATGAAATAATA-TGAAACA---TTTTAAGCACAAAAAAGTAGAGATTTAATCTCGTACCTTTTGCATCATGATTTAACTAGTTTACCCAGGCAAAACGA-ATTTAAGTTTGGCCCCCCGAAACTAAGCGAGCTACTTCAAGGCAGTTTTATGAACTCATCCGTCTCTGTTGCAAAAGAGTGGAAAGACCTTCAAGTAGGGGTGACAGACCTAACGAGCCTAGTGATAGCTGGTTACTCAAGAAAAGGATTTTAGTCCTCCCTAACGTCTATTAGA----TATCACAAAATAAAATTTAGACTTTAGAGTTATTCAATTAAGGTACAGCCTATTTGAATCAGGATACAACCTCAACTAATGGGTAAGCTCAATTT---TCTTGTATCTAAGTGGGCCTAAGAGCAGCCACCTATAA-AATAGCGTCAAAGCTTAACTACAT-TTTTCTTTAATACCTATATTTATTACCAACCCTTTACTTGTAATGAGTACTTCCATATTTTTTATGGAACTTATTATGTTAAAACTAGTAACAAGAATTAGATTTTCTCTTTAATGCAAGTGTATATCAGAAAGGACACTCCACTGACTATTAACATCCATGAATTTAG-GGTAGTAACCAAACAAGAAAACTCTACCTTCCCCATTGTTAATCTAACACAAGAGCATTTCAAGAAAGATTAAAAGGAAAAGAAGGAACTCGGCAAATATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGCATCTCTATAAGAGGTCCAGCCTGCCCAGTGAC-CCAGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCACCACGAGGGTTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTTTATTATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATGACAAATGCTTCTTT-TTTAC-TACATCCAGATAATTAATTATTAATTTAGCATTTTGACCTATAAATTTTAGGTTGGGGTGACCATGGAGCAAAAGTTAACCTCCACATTGAAAAAACCT-CATTTTTAAGCTAAAAGTTACCGCTTTAAGCATCAATATATTGACATTAATTGACCC-ATA--ATTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTGGGTTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT----------------------------------------------------------------------AACCCTGTACTTAGTGTTTGGGGCATGAGCCGGAATGGTTGGAACCGCCCTTAGTTTACTCATTCGAGCCGAATTAAGCCAACCAGGTTCTCTGCTAGGAGACGACCAGATTTATAATGTTATCGTAACTGCCCATGCCTTTGTAATGATTTTTTTTATGGTTATACCCATTATAATTGGGGGATTTGGCAACTGATTAGTTCCTCTAATAATTGGAGCCCCTGATATAGCATTCCCTCGTATAAACAATATAAGCTTTTGACTTCTGCCCCCCTCATTTCTTCTTCTCCTAGCCTCCGCCGGAGTGGAAGCTGGGGCTGGAACTGGCTGAACTGTCTACCCCCCTCTAGCCGGAAATATGGCCCATGCCGGACCGTCCGTAGACTTAACAATCTTTTCCCTTCATCTTGCTGGAGTCTCATCAATCTTAGGTGCGATCAACTTTATTACTACGATTTTAAATATGAAACCCCCTTCTGTGACCCAATACCAAACCCCCTTATTTGTCTGATCCGTTCTTATTACAGCTGTCCTTTTACTTCTTTCTTTACCTGTCTTGGCCGCCGGGATTACTATACTCCTCACAGACCGCAATTTAAATACTACCTTTTTTGACCCGGCAGGCGGGGGAGATCCCGTCCTGTACCAACATTTATTC-------
Crossodactylus_werneri ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGTGAC-CCAGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTCTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAAAATATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATAACAAATGCTTCTTTATCTACACACATTCAGATGACCAACTACTAATTCAGCATTTTGACTTATAAATTTTAGGTTGGGGTGACCGTGGAGCAAAAATTAACCTCCATATTGAAAAAACC--TCATTTTAAGCAAAAAGTTACCACTTTAAGCATCATTACACTGACATTAATTGACCCAATA--ATTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTAGGTTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAA---------------------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_amnicola --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAGCCCTTCGCTTACACCGAAAAATTATCTGTTAAACCCAGATTATTTTGAGCTAAAAATCTAACCCCCACCNTATATAATGTCATCT-CAAAANTCTTAATCATTNAATTAAAACATTCTTATAATTTAGTAGAGGCGATCAAAAAAT-TAATTGGAGTAATAAATATTGTACCGCAAGGGAAAGATGAAATAAAAATGAAAAAT--TATNAAGCACAAAAAAGTAAAGATTTACCCTTGTACCTTTTGCATCATGATTTAACTAGCTTACCCCAGCAAAACGA-CATTAAGTCGGACCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCCCATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGCGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTACTCAAGAAAAGGATTTTAGTCCCGCCTAAAGCAAACACTATTA-CTTTAAAATAAAAATTTNCGGCTTTAGAGCAATTCAATTAAGGTACAGCCTATTTGAAACAGGATACAACCTCGACTAACGGGTAACTATTACCTA--CTTCATTCTTAAGTGGGCCTAAAAGCAGCCACCTTTAATAATAGCGTCAAAGCTTTACTATGC-AAATATTTAATACCAATTTTTCTTCATAACCCTTTATTAGTACTGAGTAAATCTATATAA-CTATAGAAACTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TATATGCAGGTGTACATCAGAAAGGACATACCACTGATAATTATCACTCATGAACCCAA-AGAAGCAACTTAGCTAGAAAATTCTTCTACACTTAATGTTAATCTAACACAAGCGCATATTAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACATAAGCCTCGCCTGTTTACCAAAAACATCGCCTCTTGC--AAATTTAAGAGGTCCAGCCTGCCCAGTGAC-TCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGCTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAGCTTATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAATAACACCTGCTAATCCTCTTAC-CATATTCAGATACTCTATTTATACCTTAGCACCATGA-TTATAAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAACTAACCTCCACACTGAAGGAACT---CTTTCTAAGCAAAAAGCTACAACTTTAAGCATCAACAAATTGACACTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAAC----------------------------------------------------------------------------------AACCCTATATCTTGTATTTGGAGCATGAGCTGGGATAGTGGGAACTGCCCTAAGCCTACTCATCCGAGCCGAACTGAGCCAACCTGGCTCTCTCTTAGGTGACGACCAAATCTACAATGTTATCGTTACCGCCCACGCTTTCGTTATAATCTTTTTTATAGTAATGCCCATTATGATCGGGGGGTTCGGTAATTGACTGGTCCCCCTAATAATCGGAGCCCCGGATATAGCCTTTCCACGGATAAATAACATAAGCTTTTGACTCCTTCCCCCATCCTTTCTTCTTCTTTTAGCTTCCGCCGGAGTTGAGGCAGGCGCAGGAACAGGGTGAACAGTCTATCCCCCTCTTGCCAGCAACTTAGCCCATGCCGGACCCTCTGTAGATCTTACCATTTTTTCTCTTCACCTTGCCGGTGTTTCATCTATCCTTGGCGCCATTAACTTCATCACCACAATCCTAAATATGAAACCCCCATCTGTAACCCAGTATCAAACCCCCCTTTTTGTGTGATCTGTCTTAATTACTGCTGTTCTCCTTCTCTTATCCCTTCCGGTCTTAGCCGCTGGTATCACGATGCTTTTAACAGATCGAAATTTAAATACAACATTTTTTGACCCGGCAGGTGGGGGAGACCCCATTCTATATCAACACTTATTTTGAT---
Hylodes_asper ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAATGGCATCACGAGGGTTGAACTGTCTCCTTTCTCTAATCAGTGAAACTAATTTTCCCGTGAAGAAGCGGGAATAATTCTATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ACATAACACTTGCTAT--TACCTAT-AATCTCCCGATATTCAATCTTTATCCAAGCATNATGA-TTATTAATTTTAGGTTGGGGCGACCACGGAGCAAAAATTAACCTCCACATTGTAAAAATT----TTTTTAAGCAAAAAGTTACAACTTTAAGCATCAATAAATTGACATTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGG----------------------------------------------------------------------------------------------------------------------------------CCACAAACCACAAAGATATCGGAACCCTATATCTTGTTTTCGGGGCATGAGCCGGAATAGTCGGAACAGCCCTTAGCCTCCTAATTCGAGCTGAATTAAGCCAACCCGGATCCCTCTTAGGAGACGACCAAATTTATAATGTGATTGTTACCGCACATGCCTTTGTAATAATTTTTTTTATGGTCATACCAATTATAATTGGAGGATTTGGAAACTGACTAGTACCTTTAATAATTGGGGCCCCCGATATGGCTTTTCCTCGGATAAACAATATGAGCTTTTGATTGCTCCCACCATCTTTTCTTCTTCTTCTAGCCTCTGCCGGAGTAGAAGCAGGTGCAGGAACGGGCTGAACAGTTTACCCCCCTCTAGCTAGCAATTTAGCCCATGCAGGGCCCTCCGTTGACCTAACTATTTTCTCTCTTCATTTAGCTGGTGTCTCCTCTATTCTTGGTGCCATTAATTTCATCACAACTATTTTGAATATGAAGCCCCCNTCTATAACTCAGTATCAAACCCCACTCTTTGTTTGGTCCGTCCTTATTACCGCTGTTCTTCTCCTTCTCTCTCTCCCAGTTCTAGCCGCTGGAATCACTATGCTTCTCACTGATCGAAATTTAAACAC--------------------------------------------------------------
Hylodes_caete ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCCCTTCGCTTACACCGAAAATTTATCTGTTAAACTCAGATTATTTTGAGCTAAAAATCTAA-CCCCACCTTATTTTATGCAACCC-TAAAACT-TTAATCATCTAATTAAAACATTTTTACAGTTTAGTAGAGGCGATCAAAAAAT-TAATTGGAGTAATAAATA-TGTACCGCAAGGGAAGGATGAAATAAAAATGAAA-----ACTTAAGCACAAAAAAGTAAAGATAAACTCTTGTACCTTTTGCATCATGATTTAACTAGTCTACCCCAGCAAAACGA-CTTTAAGTCAGGCCCCCCGAAACTAAGTGAGCTACTTCAAGACAGCTTAATGAGCACATCCGTCTCTGTTGCAAAAGAGTGGAGAGATCTTCAAGTAGAGGTGACAGACCAAACGAACTAAGTGATAGCTGGTTACTCGAGAAAAGGACCTCAGTCCCACCTAAAGTTAGCAATATTA-ATTTTAAACAATAATTTTCGGCTTCAGAGTAATTCAATTAAGGTACAGCCTATTTGAAACAGGGTACAACCTAAACTAACGGGTAATT-TGATATA--CCTAATTTTTAAGTGGGCCTAAAAGCAGCCACCTTTTA-AATAGCGTCAAAGCTTTATTATAT-TTGTATTTAATACCGAATCTGTTATTTAACCCTCTATTAGTATCGAGTAAATCTATATGC-TTATAGAAATTTTTATGTTAAAACTAGTAACAAGAAATAGATTTCCTC-TGTATGTAAGTGTATATCAGAAAGGACATACCACTGATCATTAACATTCATGAGCCCAA-AGCAGTAACTTAACTAGAAAACTCTTCTATATAAAATGTTAACCTAACACGAGCACATATCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACACTAACCCCGCCTGTTTACCAAAAACATCGCCTCTTGTA-AAATTTAAGAGGTCCAGCCTGCCCAGTGAC-CCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTATTATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAACAACAATTGCTAACCTATTTAC-AATACTTAGGTATCTAATCTAAATCCTAGCATTATGA-TTTTAAGTTTTTGGTTGGGGTGACCACGGAGTAAAAATTAACCTCCATATTGGAAGAATC--TCTTTCTAAGCAAAAAGTTACTACTTTAAGCATCAACAAATTGACATCCATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCATTTTAAGAGCTCTTATCGACAAATGGGTTTACGACCTCGATGTTGGATCAGGGTACCCCCGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAGT----------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_charadranaetes ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TATCAAAAACATCGCCTCTTGTA-AAATCTAAGAGGTCTAGCCTGCCCAGTGACTTTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTGTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTATTATATAAGACGAGAAGACCCTATGGAGCTTCAAAC-AAATTACATTTGCTATT-TTTCTAC-AATCTTCCGATATTTAAATTTTATCCTAGCATTATGT-CTATTAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCATATTGAAAGATTT---ATTTCTAAGCAAAAAGTTACAACTTTAAGCATCAATAAATTGACATTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCATTTTAAGAGCTCTTATCGACAAATGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAG-----------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_fredi -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCTTAA-CTAAAGCCTTTCGCTTACACCGAAAAATTATCTGTTAAACCCAGATTATTTTGAGCTAAAAATATAA-CCCCACTTTCTTTTATATCCTT--ACAAATT-TAAATTTTAAAATTAAAACATTCTCAAAGTCAAGTAGAGGCGATCAAAAAAT-TAATTGGAGTAATAAACAATGTACCGCAAGGGAAAGATGAAATAAAA-TGAAATAA--TATCAAGCACAAAAAAGTAAAGATTAACTCTTGTACCTTTTGCATCATGATTTAACTAGTATACCCCGGCGAAACGATTTTTAAGTCGGACCCCCCGAAACTAAGTGAGCTACTTCAGGGCAGCTTTTTGAGCATATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGGGGTGATAAACCAAACGAACTAAGTGATAGCTGGTTACTCGAGAAAAGGATCTTAGTCCCGTCCAAAGCAAACATTATTA-ATTAAAAATAAAAATTTTTAGCTTTGAAGTAATTCAATTAAGGTACAGCCTACTTGATACAGGAGACAACCTAAACTAATGGGTAATTTAAATACA---TCTTTTTTCAAGTAGGCCTAAAAGCAGCCACCTTTAA-AATAGCGTCAAAGCTTCACTACAT-ACGTATTTAATACCAAACTCATCACTTAACCCTTTATTAGTATCGAGTAGATCTATATAG-TTATAGAAATTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TATATATGAGTGTACACCAGGAAGGACACACCACTGACCATTAACATCTTTGAACCTAA-AGCAGCAACTT-ACAAGAAAATTCTTCTTTACCAAATGTTGATCTAACACAAGCATATATCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACACTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTA-AAATTTAAGAGGTCTAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTCTTTCTAATCAGTGAAACTAATCTTCCTGTGAAGAAGCGGGAATTATTATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAACAACACTTGCTAATTTATTTGT-GATACTCAGGTCACCAACTTTAAACCTAGCATTATGATTTTTAAGTTTTTGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCATGCTGAAAGAACT----TCTCTAAGCAAAAAGTTAATACTTTAAGCATCAAAAAATTGACATTCATTGACCCAATA--TTTTGATTAACGAACCCAAGTTACCCTAGGGATAACCAGGGCCAATTC------------------------------------------------------------------------------------------------------------------------------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_lateristrigatus ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAAAAACATCGCCTCTTGT--AAATTTAAGAGGTCTAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTACACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAACATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAGTAGCACTTGCTAAT-TAATTAT-TATACTCAGGTATCTAACTCTTATCCTAGCACTACTGCCTACAAGTTTTCGGTTGGGGTGACCGCGGAGTAAAAACCAACCTCCACACTGAAAGAACT---CTTTCTAAGCAAAAATCTACAACTTTAAGCATCAATAAATTGACATCTATTGACCCAATA--CTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTAAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGCAGCCGCTACTGAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGA------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_meridionalis -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCTTAA-TTAAAGCCTTTCGCTTACACCGAAAAACTATCTGTTAAATCCAGATTATTTTGAGCTAAAAATCTAA-CCCCACTTTATGTTATGCTATAT-GTAAACT-TTAATCATTTAATTAAAACATTTTTACAACTTAGTAGAGGCGATCAAAAAAT-TAATTGGAGTTATAAATTATGTACCGCAAGGGAAAGATGAAATAAAAATGAAAA----CATCAAGCACAAAAAAGTAAAGATTAAGTCTTGTACCTTTTGCATCATGGTTTAACTAGCTTACCCTAGCGAAACGA-TTTTAAGTCAGACTCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCATATCCGTCTCTGTTGCAAAAGAGTGGTGGGACCTTCAAGTAGAGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTGCTCGAGAAAAGGATTTTAGTCCCGCCCAAAGCAAACATTATTATTTTTTTAAACAAAATTTTTAGCTTTAGAGTAATTCAATTAAGGTACAGCCTATTTGAAACAGGATACAACCTTGCCTAATGGGTAAA--TAACATG--CTACATCTTTAAGTAGGCCTAAAAGCAGCCACCTTTTA-AAAAGCGTCAAAGCTTCGCTATGT-TAATATTCAATACCAACCTCACAAATTAACCCTTTATCGTCATTGAGCAAATCTATACAT-CTATAGAAACTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TTTATGTAAGCTTAAATCAGAATGGACACACCACTGATAATTAACACCTATGAATCGAA-AGTAGCAACCCAACAAGAAAACTCTTCTGTACTCAATGTTAATCTAACACAAGCACATATTAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAATATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTCGTA-GAATTTGAGAGGTCCAGCCTGCCCAGTGAC-CTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTTTCTCTAATCAGTGAAACTAATCTTTCCGTGAAGAAGCGGGAATTATCCTATAAGACGAGAAGACCCTATGGAGCTTTAAAC-TAATAACACCTGCTAATTTAAATAT--TTATTCTGATTTTTAACCTATATTCTAGCACAATGA-TTAAGAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCACATTGAATTAGAG--CATATCTAAGCAAAAAACCACATCTTTAAGCATCAACAAACTGACATTTATTGACCCAATA--ATTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTACCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT-----------------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_nasus --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAGCCCTTCGCTTACACCGAAAAGTTATCTATT-AATACAGATTATTTTGAGCCAACAATCTAA-CCCCACTTCACCCTATAACATCT-CAACACT-TTTGTGATAAAATCAAAACATTTTAATAACTTAGTAGAGGCGATCAAAAAGT-TAACTGGAGTAATATCAAATGTACCGCAAGGGAAAGATGAAATAAAAATGAAATATC-TTCCAAGCACAATAAAGTAAAGATTCTCTCTTGTACCTTTTGCATCATGATTTAACTAGTCTACCTCAGCAAAATGA-CTTTTAGTCTAGCCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCATATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGAGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTACTCGAGAAAAGGATCTTAGTCCCACCTAAAACTATTG-TATTT-TTATAAAATAAAAATTTATAACTTTAGAGTAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTTTACTAATGGGTAAAC-TTCTACT--CACAATTTTTAAGTGGGCCTAAAAGCAGCCACCTTTCA-AAAAGCGTCAAAGCTTTACTATCG-TGATAAAAAATATCAATTTTGTTACACAACCCTTTACTAGTACCGAGTAATTCTATATTA-CTATAGAAATTTTTATGTTAAAACTAGTAACAAGAATAAAATTTTCTC-TGTATGTAAGTGTACACCAGGAAGGACAAACCACTGGTAATTAACATTCGTGAATCTAATAGAAGCAACTTAACAAGAAAAACCTTCTATAACCCATGTTAATCTAACACAAGCACATGTCAAGAAAGATTAAAAGGAAAAGAAGGAACTCGGCAAACATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTA-AAATTTTAGAGGTCCAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTTTTCCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATAATTTTATAAGACGAGAAGACCCTATGGAGCTTTAAAT-GAATAACACCTGCTAAC-TATCTAC-AATATTCAGATACTTAACTTATATTTTAGCATGATGA-TTACTAGTTTTAGGTTGGGGTGACCACGGAGTAAAAATTAACCTCCATATTGTAAAGAAT---AATTTTAAGCAAGAAGTTACAACTTTAAGCATCAATAAATTGACATTTATTGACCCAATA--TTTTGAGCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAAC------------------------------------------------------------------------------------CCCTTTATCTTGTTTTCGGAGCATGAGCCGGAATAGTCGGAACTGCTCTAAGCCTTCTTATCCGTGCCGAACTAAGCCAACCTGGGTCGCTCTTGGGAGACGACCAAATTTACAATGTGATTGTTACCGCCCATGCTTTCGTGATAATTTTTTTTATAGTTATACCCATTATGATCGGAGGGTTTGGAAACTGACTGGTGCCCCTAATGATTGGAGCCCCCGACATGGCCTTCCCTCGAATAAACAACATAAGTTTCTGACTTCTTCCTCCATCTTTTCTTCTTCTTTTAGCCTCCGCCGGAGTAGAAGCAGGTGCAGGAACAGGGTGAACAGTCTACCCCCCCTTAGCTAGCAATCTCGCTCATGCAGGTCCATCCGTGGATCTAACTATCTTCTCTCTCCACCTAGCTGGTGTCTCATCTATTCTTGGGGCCATTAACTTTATTACCACAATTCTTAACATGAAACCACCCTCAATGACCCAGTATCAGACCCCACTTTTTGTTTGATCTGTCCTTATCACTGCAGTTCTTCTGCTCCTTTCCCTCCCAGTCCTAGCCGCTGGCATCACTATGCTACTCACAGACCGAAATCTAAACACAACATTCTTTGACCCGGCAGGAGGGGGAGATCCTATTCT-----------------------
Hylodes_ornatus --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAGCCCTTCGCTTACACCGAAAATTTATCTGTTAGACCCAGATTATTTTGAGCTAAAGATCTAA-CCCCACCTTATTTTATGTCATCT-CAAGACT-TTAATCATTTAACTAAAACATTCTTATAGTTTAGTAGAGGCGATCAAAAAAT-TAATCGGAGTAATAAATATTGTACCGCAAGGGAAAGATGAAATAAAAATGAAAAAT--TTTTAAGCACAAAAAAGTAAAGATTCACCCTTGTACCTTTTGCATCATGATTTAACTAGCTTACCCCAGCAAAACGA-TATTAAGTCGGACCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCATATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGCGGTGATAGACCTAACGAACTTAGTGATAGCTGGTTACTCAAGAAAAGGATTTTAGTCCCGCCTAAAACAAACATTATTA-TTTTAAAATAAGAATTTTCGGCTTTAGAGCAATTCAACTAAGGTACAGCCTATTTGAAACAGGATACAACCTTGACTAATGGATAAC--TTATATA--CTTCATTTTTAAGTGGGCCTAAAAGCAGCCACCTTTAATAATAGCGTCAAAGCTTTATTATGT-AAATATTTAATACCAATTTTTTTTCATAACCCTTTATTAGTACTGAGTAAATCTATACAA-TTATAGAAACTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TATATGCAGGTGTATATCAGAAAGGACATACCACTGATAATTATCATTCGTGAACCCAA-AGAAGCAACTTAACTAGAAAACTCTTCTATATTTAATGTTAATCTAACACAAGCGCATGTCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACATTAGCCTCGCCTGTTTACCAAAAACATCGCCTCTTGC--AAATTTAAGAGGTCCAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGCTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAATCTATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAATAACATTTGCTATC-CTTTTAC-AACATTCAGATATCTAATCTTTACCTTAGCACTATGA-TTACAAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCACATTGAAGGAACT---CTTTCTAAGCAAAAAGCTACAACTTTAAGCATCAACAAATTGACACTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCATTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAAC-----------------------------------------------------------------------------------ACTCTATATCTTGTGTTTGGAGCATGAGCTGGGATAGTCGGAACTGCCCTAAGCTTACTCATCCGAGCTGAACTTAGCCAGCCCGGCTCCCTCTTAGGGGACGATCAAATTTACAACGTCATCGTCACCGCACATGCCTTCGTTATAATCTTCTTTATAGTCATGCCCATCATAATCGGCGGGTTCGGTAACTGACTGGTTCCCCTAATAATTGGAGCCCCGGACATGGCCTTTCCCCGAATAAATAATATAAGCTTTTGACTCCTTCCACCATCCTTTCTTCTTCTTTTGGCCTCCGCAGGTGTTGAAGCTGGCGCAGGAACAGGGTGGACAGTCTACCCCCCTCTTGCTAGCAACTTAGCCCACGCCGGACCCTCTGTAGATCTTACTATCTTTTCCCTTCACCTCGCCGGTGTATCGTCCATTCTTGGTGCCATTAATTTTATCACCACGATCCTAAATATGAAGCCCCCATCTGTAACCCAGTATCAAACTCCCCTTTTTGTGTGGTCTGTTTTGATCACTGCTGTTCTTCTTCTATTATCCCTTCCAGTATTAGCCGCTGGCATCACAATGCTTTTGACAGATCGAAATCTAAATACAACATTCTTTGACCCGGCAGGTGGAGGAGACCCCATTCTTTAC-------------------
Hylodes_perere ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????--------------------------------------------------------------------------------------GGGAACTGCCCTAAGCCTGCTCATCCGAGCCGAACTAAGCCAGCCTGGCTCACTCCTAGGGGACGATCAAATTTACAATGTTATCGTTACCGCACACGCTTTTGTTATAATTTTTTTTATAGTAATGCCCATTATGATCGGTGGGTTTGGTAATTGATTGGTCCCCTTAATAATTGGAGCCCCAGATATAGCCTTTCCACGGATAAATAACATGAGCTTTTGACTCCTTCCCCCATCCTTTCTTCTTCTTTTAGCCTCCGCCGGAGTTGAGGCGGGCGCAGGGACAGGGTGGACAGTCTACCCCCCTCTTGCCAGCAACTTAGCCCATGCCGGACCCTCTGTAGATCTAACCATTTTTTCCCTTCACCTTGCCGGTGTGTCATCCATCCTTGGTGCCATTAATTTCATCACTACAATCCTGAACATAAAACCCCCATCTGTAACCCAGTATCAAACCCCCCTTTTTGTGTGATCTGTTTTAATTACTGCTGTTCTCCTTCTCTTATCCCTTCCGGTCTTAGCCGCTGGTATCACAATGCTTTTAACAGATCGAAACTTAAACACAACATTTTTTGACCCGGCAGGTGGGGGGGACCCCATTCTCTAC-------------------
Hylodes_phyllodes -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGTATACCGGAAGGTGTGCTTGGAAACAAAGTATAGCTTAA-CCAAAGCCTTTCGCTTACACCGAAAAACTATCTGTTAAACCCAGATTATTTTGAGCCGAAAACCTAA-CCCCACCACCCCTTTTATATCCCACAAAACC-TTAATTATTCTACTAAAACATTTTCACAGCTTAGTAGAGGCGATCAAAAAAC-TAATTGGAGTAATAACTTTTGTACCGCAAGGGAAAGATGAAATAAAATTAAAA-----CTTAAAGCACAAAAAAGTAAAGATTAACCCTTGTACCTTTTGCATCATGATTTAACTAGTCTTCTTCGGCAAAACGATTTTTAAGTCGATTCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCACACCCGTCTCTGTTGCAAAAGAGTGGAAAGACCTTCAAGTAGAGGTGACAGACCAAACGAACTTAGTGATAGCTGGTTACTCGAGAAAAGGATTTAAGTCCCCCCTAAAGCAAACACTATTA-ACTAAAAATAATAATTT--AGCTTAAGAGCAATTCAATTAAGGTACAGCCTACTTGAAACAGGACACAACCTCAACTAACGGGTAATTTCAGTACA--CCTTATTTTCAAGTAGGCTTAAAAGCAGCCACCTTTCA-GATAGCGTCAAAGCTTTATTATAC-ATGTACTCAATACCTAATCTGTTATTTAACCCTTTATTAATATCGAGTAAATCTATAAAACCTATAGAAACTTTTATGTTAAAACTAGTAACAAGAACTAGACTCTCTC-TATATGTAAGTATACGTCAGAAAGGACCCTCCACTGACTATTAACACCCATGAACAAAA-AGAAGTAACTCAACAAGAAAACTCTTCTACACCAAGTGTTAATCTAACACAAGCACATATTAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACACCAACCCCGCCTGTTTACCAAAAACATCGCCTCTTGTA-AAATTTAAGAGGTCTAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTCCCCGTGAAGAAGCGGGGATCAATATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAACAACAACTGCTAATTTATTTAT-GACACTCAGGTTCCTAATCTTAACCCTAGCACTATGA-TCTTAAGTTTTTGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCACATTGAAAGTAAT--CCTTTCTAAGTAAAAAGTTACTACTTTAAACATCAACAAATTGACATTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCATTTTAAGAGCCCCTATCGACAAATGGGTTTACGACCTCGATGTTGGATCAGGGTATCCAAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAGT---------------------------------------------------------TACCTTATACCTCGTGTTTGGGGCATGAGCTGGGATAGTGGGGACCGCTCTTAGCCTCTTAATCCGAGCCGAGCTTAGCCAGCCCGGCTCGCTTCTGGGGGATGACCAAATTTATAACGTGATCGTCACTGCTCACGCCTTTGTAATAATCTTTTTTATAGTAATGCCGATTATAATCGGCGGATTTGGCAATTGACTGGTCCCATTAATAATTGGGGCCCCCGACATAGCCTTCCCTCGAATAAACAACATAAGTTTCTGACTCCTCCCTCCATCTTTTCTCCTCCTATTAGCCTCAGCTGGGGTCGAGGCAGGCGCCGGAACGGGGTGGACAGTATATCCGCCTCTTGCTAGCAACTTAGCACACGCCGGGCCATCTGTGGACCTCACTATTTTTTCTCTCCACTTGGCCGGGGTATCTTCAATCCTGGGGGCTATCAATTTTATTACTACAATTCTCAACATGAAGCCCCCCTCTGTAACCCAGTATCAAACCCCTCTTTTTGTTTGATCTGTTCTTATCACTGCCGTTCTCCTTCTTTTATCCCTTCCAGTTCTAGCTGCCGGAATTACTATGCTCCTCACAGACCGTAACCTAAACACAACATTCTTTGACCCAGCTGGGGGCGGGGATCCCATTCTATACCAACATTTATTC-------
Hylodes_pipilans --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAGCCTTTCGCTTACACCGAAGAACTATCTGTTAAACCCAGATTATTTTGAGCTAAAAATTTAA-CCCCACCTTCTTTTATGCCCACCACAAGACT-TAAATTATATAACTAAAACATTCTTACAGTTTAGTAGAGGCGATCAAAAAAC-TAACAGGAGTAATAAATAATGTACCGCAAGGGAAAAATGAAATAAAAATGAAATAC--TATCAAGCAAAAAAAAGTAAAGATTAATCCTTGTACCTTTTGCATCATGATTTAACTAGCCTACCCCGGCAAAACGACTTTTAAGTCAGACCTCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCTTATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGAGGTGACAGACCAAACGAACTAAGTGATAGCTGGTTACTCGAGAAAAGGATCTTAGTCCCGCCCAAAGAAAACACTATTA-ATTAAAAATAAAAATTTTCGACTTTAAAGCAATTCAATTAAGGTACAGCCTACTTGATACAGGAAACAACCTAAACTAACGGATAATCATTATACACCCTCCCCTTTTAAGTGGGCCTAAAAGCAGCCACCTTTAA-AATAGCGTCAAAGCTTCATTATAC-ATGTATCTAATACCAAATTTATTATTTAACCCTTTATTAATATCGAGTAAATCTATAAAA-CTATAGAAATTTTTATGTTAAAACTAGTAACAAGAGTTAGATTTTCTC-TTTATGTAAGTGTATATCAGAAAGGACACACCACTGACTATTAACATCTATGACCTTAG-AGCAGCAACTTGACAAGAAAACCCTCCTCCCCTAAATGTTAATCTAACACAAGCACATACCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACATTAACCCCGCCTGTTTACCAAAAACATCGCCTCTTGCA-AAACTTAAGAGGTCTAGCCTGCCCAGTGAC-TCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTACACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGGATCATTATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAACAACATTTGCTAACTCACCCGC-GATACTCAGGTCGCTAATCTTAATCCTAGCACTATGA-TTTCAAGTTTTTGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCACAATGAAAGAACT-----CTCTAAGTAAAAAGCTACTACTTTAAGCATCAATAAATTGACATTCATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTAAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTACCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAAC------------------------------------------------------------------------------------CTCTGTACCTTGTATTCGGGGCATGAGCCGGAATGGTGGGAACTGCCCTAAGCCTCCTGATTCGAGCTGAACTAAGTCAGCCCGGCTCACTATTAGGAGACGATCAGATTTATAATGTTATCGTCACCGCCCATGCCTTTGTAATAATTTTTTTTATAGTAATGCCTATTATAATCGGCGGATTCGGTAACTGACTGGTACCTCTAATAATCGGAGCCCCGGATATGGCCTTTCCCCGGATAAACAATATAAGCTTCTGACTCCTCCCTCCGTCTTTTCTTCTTCTATTAGCCTCAGCTGGTGTAGAAGCAGGCGCCGGGACGGGTTGAACAGTTTATCCCCCTCTTGCTAGCAACCTAGCCCATGCCGGACCCTCTGTAGACTTAACCATCTTTTCCCTTCACTTAGCTGGTGTGTCATCTATTCTTGGCGCTATTAATTTTATTACCACAATCATCAACATAAAACCTCCTTCAGTAACCCAATATCAAACCCCACTCTTCGTTTGATCTGTCCTCATTACCGCTGTCCTTCTACTCCTATCACTCCCAGTTCTGGCTGCCGGAATCACAATGCTCCTAACAGATCGAAACTTAAACACAACATTCTTTGACCCAGCTGGAGGAGGAGATCCCGTTCT-----------------------
Hylodes_sazimai -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGCTTAA-TTAAAGCCCTTCGCTTACACCGAAAAATTATCTGTTAGACCCAGATTATTTTGAGCTAAAAATCTAA-CCCCACCTTATATTATATCATCT-CAAGACT-TTAATCATTTAATTAAAACATTCTTATAGTTTAGTAGAGGCGATCAAAAAAT-TAATTGGAGTGATAAATACTGTACCGCAAGGGAAAGATGAAATAAAAATGAAAAAT--TATTAAGCACAAAAAAGTAAAGATTTACCCTTGTACCTTTTGCATCATGATTTAACTAGCTTACCCCAGCAAAACGA-CATTAAGTCGGACCCCCCGAAACTTAGTGAGCTACTTCAAGGCAGCTTAATGAGCACATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGCGGTGATAGACCTAACGAACTAAGTGATAGCTGGTTACTCAAGAAAAGAATTTTAGTTCCACCTAAAGCAAACATTATTA-CTTTTAAATAAGAATTTCTAGCTTTAGAGCAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTTGACTAATGGGTAACTATTATATA--CCTCATTTTTAAGTGGGCCTAAAAGCAGCCACCTTTAATAATAGCGTCAAAGCTTTATTATGC-AAATATTTAATACCAATTTTTTTTCATAACCCTTTATTAGTACTGAGTAAATCTATATAA-TTATAGAAACTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TTTATGCAGGTGTATATCAGAAAGGACATACCACTGATAATTACCATTCATGAACCTAA-AGAAGCAACTTAACTAGAAAACTCTTCTATAATCAATGTTAATCTAACACAAGAGCATATCANGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAATATTAGCCTCGCCTGTTTACCAAAAACATCGCCTCTTGC--AAATCTAAGAGGTCCAGCCTGCCCAGTGAC-TCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGCTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATCGATTTATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAATAACACCTGCTAATCCACTTAT-AATATTCAGATACTTTATTTATATCTTAGCACTATGA-TTACAAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAACCAACCTCCACATTGAAAGAACT---CTTTCTAAGCAAAAAGCTACAACTTTAAGCATCAATAAATTGACACTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCT--------------------------------------------------------------------------------------------------------ACAGCTCTAAGCCTACTCATCCGAGCCGAACTAAGCCAGCCTGGCTCACTTTTAGGAGATGACCAAATTTATAATGTTATCGTTACCGCACATGCTTTTGTTATAATTTTTTTCATAGTAATGCCTATTATAATTGGTGGGTTCGGTAATTGACTGGTCCCCCTAATAATTGGAGCTCCAGATATGGCCTTTCCACGAATAAATAATATGAGTTTTTGACTCCTCCCTCCCTCCTTCCTTCTTCTTTTGGCCTCCGCCGGAGTTGAAGCAGGCGCAGGAACAGGGTGAACAGTCTACCCCCCTCTTGCCAGCAACTTAGCCCATGCTGGGCCCTCTGTAGATCTCACCATTTTTTCTCTTCACCTTGCCGGCGTATCGTCCATCCTTGGTGCCATTAATTTTATCACTACGATTCTAAACATAAAACCCCCATCCATAACCCAATATCAAACCCCCCTTTTTGTCTGATCTGTTTTAATCACTGCTGTTCTTCTTCTCTTATCCCTTCCGGTTTTAGCCGCTGGTATCACAATGCTTCTCACGGATCGAAATTTAAATACAACATTTTTTGACCCGGCAGGTGGAGGGGACCCCATTCTTTACCAACATTTATTTTGATTCT
Hylodes_uai -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGCCTGTTTATCAAAAACATCGCCTCTTGC--AAATTTAAGAGGTCTAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTACACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTACTATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAGTAACATCTGCTAAT-TAATTAT-CATATTCAGATATCTAACTTTTAACCTAGCACTATTACCTACTAGTTTTTGGTTGGGGTGACCGCGGAGTACAAAATAACCTCCACATTGAAAGAACT---CTTTCTAAGCAAAAAACTACAATTTTAAGCATCAACAAATTGACATCTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTCAAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAGTTCAGACCGG-????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Megaelosia_apuana ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????----------------------------------------------GAACCCTATACCTGGTTTTCGGGGCATGAGCCGGAATAGTCGGAACAGCCCTAAGTCTTCTAATCCGAGCCGAATTAAGCCAGCCCGGCTCCCTCCTTGGAGACGATCAAATTTATAACGTTATTGTTACCGCTCACGCTTTCGTCATGATTTTCTTCATGGTGATACCCATCATGATTGGAGGTTTTGGTAATTGACTAGTACCTCTTATAATTGGGGCCCCTGATATGGCCTTCCCTCGAATAAACAACATAAGTTTTTGACTTCTACCCCCGTCCTTTCTTCTACTCCTTGCCTCCGCCGGCGTTGAGGCGGGGGCCGGCACCGGGTGGACAGTCTACCCCCCTCTTGCCGGTAATTTAGCCCATGCCGGCCCCTCTGTAGATCTAACCATCTTTTCCCTCCACCTTGCCGGTGTGTCATCAATCCTTGGGGCCATTAACTTTATCACCACAATCCTTAATATAAAACCTCCCTCAATAACCCAATATCAGACGCCTCTTTTTGTCTGATCTGTTCTGATTACAGCTGTCCTTCTTCTTTTATCCTTGCCTGTCTTAGCCGCCGGAATCACAATACTGCTTACAGACCGTAACCTAAACACAACCTTCTTTGACCCGGCTGGGGGTGGAGATCCAGTTCTCTAT-------------------
Megaelosia_boticariana -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGCTTAG-CTAAAGCACCTCGCTTACACCGAGTAAACATCTGTGAAACCCAGATTATTTTGAGCCAAAAATCTAG-CCCTATCTC-CCCCATGTAAACT-CAACATT-TCAAATTATAAATCAAAACATTTTAATGATTAAGTAAAGGCGATTGAAAAAT-TAACAGGAGCTATATACAATGTACCGCAAGGGAAAGATGAAATAAAAATGAAATATT-TATCAAGCACAAAAAAGTAAAGATTAATTCTTGTACCTTTTGCATCATGATTTAACTAGTCTACCCTAGCAAAACGA-TTTTTAGTTTGACCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCAAATCCGTCTCTGTTGCAAAAGAGTGGAAAGACCTTCAAGTAGGGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTACTCAAGAAAAGGATCTAAGTCCAACCTAAAACTTAACTTATAC-CACAAGATTAAAAACTT--AACTTTAGAGCAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTCAACTAATGGGTTATCTTAACCAC--CAGCACATTTAAGTTGGCCTAAGAGCAGCCACCTTTTA-AATAGCGTCAAAGCTTAGCCATGC-GACTGTTTAATCTCAACAACACCTATTAACCCTTTACTACTATTGAGTACTTCTATATAC-CTATAGAACTTTTTATGTTAAAACTAGTAACAAGAACTAGGCCTTCTCTTATATGTCAGTGTATATCAGTAAGGACACACCACTGATAATTAACACCAATGAACTAAA-AGTAGCAACTCAACAAGAAAACCCTTCTTAATAATATGTTAATCTAACACAAGTACATTCCAAGAAAGATTTAAAGAAAAAGAAGGAACTCGGCAAATATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTAAAAATTTAAGAGGTCCAGCCTGCCCAGTGAC-CCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAAGGTTATACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATAATTATATAAGACGAGAAGACCCTATGGAGCTTTAAACAAAATAACAATTGCTAAC-TAAATCT-TAATTTCAGAAAACCAATCTTTATCCTAGCATTATGA-TTACCAGTTTTAGGTTGGGGTGACCACGGAGAAAAAAGTAACCTCCATATTGAAAGAACTA-CCATTCTAAGCAAAAAGCTACAACTTTAAGCATCAACAAATTGACATCTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCTATCCACTTTAAGAGCCCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGCAGCCGCTACTAAAGGTTCG-TTGTTCAACGATAAAACC--------------------------GTGATAATTACCCGATGATTTTTTTCAACTAACCATAAAGATATTGGAACCCTATACCTGGTCTTCGGGGCATGAGCCGGAATAGTCGGAACAGCCCTAAGTCTCCTAATCCGAGCCGAATTAAGCCAACCCGGCTCTCTCCTCGGAGATGACCAAATTTATAATGTTATTGTTACCGCCCACGCTTTCGTCATGATTTTTTTCATGGTAATACCCATCATAATTGGAGGCTTTGGTAACTGACTCGTACCCCTTATAATTGGAGCCCCTGATATAGCTTTCCCCCGAATGAACAACATAAGCTTCTGACTTCTACCACCCTCCTTTCTTCTACTTCTTGCCTCCGCCGGCGTTGAAGCAGGGGCCGGCACCGGGTGAACAGTTTATCCCCCTCTTGCCGGTAATATAGCCCACGCTGGCCCCTCTGTAGACCTAACTATTTTTTCTCTTCACCTTGCCGGTGTGTCATCAATTCTTGGGGCCATTAATTTTATTACTACAATCCTTAATATAAAACCCCCTTCAATAACCCAGTATCAAACGCCTCTTTTTGTCTGATCTGTCTTGATCACAGCTGTCCTTCTTCTCCTGTCCCTACCCGTTTTAGCCGCCGGAATTACAATACTTCTCACAGACCGAAATGTAAACACAACCTTTTTTGACCCAGCTGG-----------------------------------------
Megaelosia_goeldii GTTTGGTCCTAACCTTGTAATCAATTTTTACTTAATATACACATGCAAGTCTCCGCACCCCTGTGAAAACGCCCTTAAATCCCCCAT--GGGATAA-GGAGCTGGTATCAGGCACGAAAATCTGCCCAAAACACCTAGCTATGCCACACCCACAAGGGTACTCAGCAGTGATTGACATTATTTATAAGCGCCAGCTTGACTCAGTTAAAGTAAAGAGAGCCGGCAAATCTGGTGCCAGCCGCCGCGGTTACCCCACGTGGCTCAAATTGATTTCTTTCGGCGTAAAGCGTGATTAAAGTGCCCATCAACATTGGAGTTAAACTAAAATTAAGCTGTGACACGCTTATTTTACAGAAAAGCACAAACGAAAGTTACTTCAATTTAAACAACTTGAATTCACGACAGTCAGGACACAAACTGGGATTAGATACCCCACTATGCCCGACCGTAAACTTTAATTTACACCAT-CACCGCCAGAGAACTACGAGCAAAGCTTAAAACTCAAAGGACTTGACGGTCCCCCACATCCCCCTAGAGGAGCCTGTCCTTTAATCGATAATCCCCGCTTAACCTCACCATTCTTAGTCTTTCAGCCTGTATACCTCCGTCGTCAGCTTACCCCGTGAGCGAAAATTAGTGAGCTTAATGTCCACACGTCTACACGTCAGGTCAAGGTGCAGCAAATATAATGGGAAGAGATGGGCTACACTTTCTAGTCTAGAATATACGAAAGACCACCTATGAAA-CCTGGTCAGAAGGCGGATTTAGAAGTAAAAGGAAACCAGAGCATCCCTTTTAATTTGGCACTGGGGCATGTACACACCGCCCGTCACCCTCTTCA-AAGCCTAATTTTAGTATCTAACCAACTAACG-CCTAGTAGAAGAGGCAAGTCGTAACATGGTAAGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCCTAA-TCAAAGCATTTCGCTTACACCGAAAAGTTATCTGTGAAATTCAGATTATTTTGAGCTAAAAATCTAG-CCCCACTTTATTCTATAATCCCT-TATCACT-TAAATTCATGAATCAAAACATTTTAATAATCAAGTAAAGGCGATTGAAAAAT-TAATAGGAGCAATATATACTGTACCGCAAGGGAAAGATGAAATAGAAATGAAATAATAATTAAAGCATAAAAAAGTAAAGATTAAATCTTGTACCTTTTGCATCATGATTTAACTAGTCTACCCAGGCAAAATGA-TTTTAAGTCTGACCTCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCAAATCCGTCTCTGTCGCAAAAGAGTGGAGAGACCTTCAAGTAGAAGTGATAGACCTAACGAACTTAGTAATAGCTGGTTATTCAAGAAAAGGATCTCAGTCCAACCTAAAGTCAAAT-TAATG-TTTAAAAATAAAAATTC-TGACCTTAGAGTAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTTAACTAATGGGTAAC--TTACCCC--TTCATCTTTTAAGTGGGCCTAAAAGCAGCCACCTTTAA-AATAGCGTCAAAGCTTAGCCGTCCTATACATCTAATACCAAAAACATCTATGAACCCTATACTCATATTGAATAATTCTATATTA-TTATAGAGATTTTTATGTTAAAACTAGTAACAAGAATTAAATTTTCTCTATTATGTTCGTGTACATCAGAAAGGATAAACCACTGATAATTGACATGCATGAGTAAAA-AGCAGTAACTTAACAAGAAAACCCTCCTAACTCTAATGTTAACCTAACACAAGTACATCTCAAGAAAGATTTAAAGAAAAAGAAGGAACTCGGCAAACATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTCAAAATTTAAGAGGTCCAGCCTGCCCAGTGAC-CCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTTTTATATAAGACGAGAAGACCCTATGGAGCTTTAGAC-GAGTAACAACTGCTAAT-TTTATAA-TAT-TTCAGATAAT--ATCTCTATCCTAGCATTATGA-TTATAAGTCTTTGGTTGGGGTGACCGCGGAGAAAAAAATAACCTCCACATTGAAAGAATA--TTATTCTAAGCAAAAAGACACATCTTTAAGCATCAACAAATTGACATCTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTCGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT----------------------------------------------------------------------AACCTTATACCTTGTATTTGGGGCATGAGCTGGTATAGTGGGCACCGCCCTAAGCCTCCTTATTCGAGCTGAATTAAGCCAACCCGGCTCTTTACTCGGAGACGACCAAATTTATAACGTCATTGTTACCGCCCACGCCTTTGTTATAATCTTCTTCATAGTAATACCAATCATGATTGGAGGTTTCGGTAACTGACTTGTGCCACTAATAATTGGAGCCCCCGACATAGCTTTCCCTCGAATAAATAATATAAGCTTTTGACTCCTGCCCCCATCTTTTCTTCTTCTTCTCGCCTCTGCCGGCGTTGAAGCGGGCGCTGGCACAGGCTGGACAGTATACCCGCCACTTGCGGGAAACCTAGCTCACGCCGGCCCCTCTGTTGACCTTACCATTTTTTCTCTTCATCTCGCCGGTGTTTCATCTATTCTTGGAGCCATTAATTTTATTACGACAATCCTTAATATAAAACCCCCATCAATAACCCAATACCAAACACCTCTTTTCGTCTGATCCGTCCTTATCACTGCCGTTCTCCTTCTCTTATCTCTTCCTGTCTTAGCAGCAGGCATCACCATGCTTCTTACAGACCGCAACTTAAATACAACATTCTTTGATCCTGCAGGAGGGGGGGACCCAGTTCTTTATCAACATCTGTTT-------
Megaelosia_jordanensis GTTTGGTCCTAACCTTGTAATCAATTCTTACTTAATATACACATGCAAGTCTCCGCCTCCCTGTGAAAACGCCCTTAAA-TCCCCATTAGGGATAA-GGAGCCGGTATCAGGCACGATAATCTGCCCAAAACACCTAGCTATGCCACACCCACAAGGG-ACTCAGCAGTGATTAACATTGAACATAAGCGTCAGCTTGACTCAGTTAAAGTAAAGAGAGCCGGCAAATCTGGTGCCAGCCGCCGCGGTTACCCCACGTGGCTCAAATTGATTTCTATCGGCGTAAAGCGTGATTAAAGTCCCCATT-ATATTGAAGTTAAACTGAAACTAAGCTGTGACACGCTTGTTACACAGAAAACCATAAACGAAAGTTACTTCAACTTT-TCAACTTGAATACACGACAGCTAGGGCACAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTAATTACACCCCCCAACGCCCGAGAACTACGAGCAAAGCTTAAAACTCAAAGGACTTGACGGTACCCCATATCCCCCTAGAGGAGCCTGTCCTATAATCGATAATCCCCGTTTAACCTCACCACTTTTAGTTTTTCAGCCTGTATACCTCCGTCGTCAGCTTACCACGTGAGCGAAATTTAGTGAGCTTAATGTCCACACGCCCACACGTCAGGTCAAGGTGCAGCAAATGAAGTGGGAAGAGATGGGCTACACTTTCTATATTAGAAAACACGAAAGACCACACATGAAA-CTTGGTCAGAAGGCGGATTTAGTAGTAAAAAGAAATCAAAATGTTCTTTTTAATTCGGCACTGGGGTGTGTACACACCGCCCGTCACCCTCTTCATAAGCCTAACTCTAGTATTTAACCAACTTATGCCCGAATAGAAGAGGCAAGTCGTAACATGGTAAGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCTTAA-CTAAAGCACCTCGCTTACACCGAGTAAACATCTGTGAGACCCAGATTATTTTGAGCCAAAAATCTAG-CCCCTCTCC-CCCCATGTAAACT-CAACATT-TCAGATTATAAACTAAAACATTTTAATAATAGAGTAAAGGCGATTGAAAAAT-TAACAGGAGCTATATATAATGTACCGCAAGGGAAAGATGAAATAAAAATGAAATATT-TATTAAGCACTAAAAAGTAAAGATTAACTCTTGTACCTTTTGCATCATGATTTAACTAGTTTACCCTAGCAAAACGA-TTTTTAGTTTGACTTCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCAAATCCGTCTCTGTTGCAAAAGAGTGGAAAGACCTTCAAGTAGGGGTGACAGACCTAACGAGCTTAGTGATAGCTGGTTACTCAAGAAAAGGATCTAAGTCCAACCTAAAGCTTAATTTAACA-CCACAAATTAAAAACTT--AACTTTAGAGCAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTCAACTAATGGGTTATTTTAACCAT--CAACACATTTAAGTTGGCCTAAGAGCAGCCACCTTTTA-AATAGCGTCAAAGCTTAGCCATGC-AACTGTTTAATCTCAACAACACCTTTTAACCCTTTACTACTATTGAGTACTTCTATATAC-CTATAGAACTTTTTATGTTAAAACTAGTAACAAGAACCAGACCTTCTCTTATATGTCAGTGTACATCAGTAAGGACACACCACTGATAATTAACACCAATGAACTAAA-AGTAGCAACTCAGCAAGAAAACTCTTCTTAACAATATGTTAATCTAACACAAGTACATTCCAAGAAAGATTTAAAGAAAAAGAAGGAACTCGGCAAACATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTAGAAATTTAAGAGGTCCAGCCTGCCCAGTGAC-TCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAAGGTTACACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATAATTATATAAGACGAGAAGACCCTATGGAGCTTTAAACAAAGTAACAACTGCTAAC-TAAATCT-TAATTTCAGAAAACCAATCTTTAACCTAGCATTGTGA-TTACTAGTTTTAGGTTGGGGTGACCGCGGAGCAAAAAACAACCTCCATATTGAAAGAACTA-TAATTCTAAGCAAAAAGCTACAACTTTAAGCATCAACAAATTGACATCTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCTATCCACTTTAAGAGCCCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTACCCCGGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT-----------------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
;
END;
Uma das vantagens do formato nexus é que é um formato em blocos. Este acima é o bloco que contem sua matriz (é chamado data block), e ele começa em
BEGIN DATA; e termina em END;. Então, ao invés de escrevermos todos os comandos no console do MrBayes, os escreveremos num próximo bloco.
Primeiramente, abra o arquivo best_scheme.txt que fizemos com o PartitionFinder2 na aula anterior. Qual foi mesmo nosso esquema de partições que mais se encaixa nos nossos dados? Quais os modelos de substituição nucleotídica que mais se encaixam em cada uma das partições?
Vamos preparar nosso bloco com os comandos do MrBayes com base nos resultados do PF2. Num editor de texto, adicione o seguinte no final do arquivo hylodidae.nex:
begin mrbayes;
outgroup Limnomedusa_macroglossa;
charset h1 = 1-2475;
charset coi_pos1 = 2476-3187\3;
charset coi_pos2 = 2477-3187\3;
charset coi_pos3 = 2478-3187\3;
partition PartitionFinder = 4:h1, coi_pos1, coi_pos2, coi_pos3;
set partition=PartitionFinder;
lset applyto=(1) nst=6 rates=invgamma;
lset applyto=(2) nst=6 rates=propinv;
lset applyto=(3) nst=1 rates=propinv;
lset applyto=(4) nst=6 rates=gamma;
prset applyto=(all) ratepr=variable;
unlink statefreq=(all) revmat=(all) shape=(all) pinvar=(all) tratio=(all);
Em vez de explicarmos cada um desses parâmetros aqui, porque não abrimos o MrBayes e deixamos ele nos dizer o que cada um significa? Digite o comando help
seguido de um outro comando, como por exemplo:
MrBayes > help outgroup
---------------------------------------------------------------------------
Outgroup
This command assigns a taxon to the outgroup. The correct usage is:
outgroup <number>/<taxon name>
For example, "outgroup 3" assigns the third taxon in the matrix to be
the outgroup. Similarly, "outgroup Homo_sapiens" assings the taxon
"Homo_sapiens" to be the outgroup (assuming that there is a taxon named
"Homo_sapiens" in the matrix). Only a single taxon can be assigned to
be the outgroup.
---------------------------------------------------------------------------
Caso você não tenha o MrBayes instalado e quiser fazê-lo, você pode baixá-lo aqui.
O programa está nos dizendo que o comando outgroup define uma raiz. Agora faça com os outros comandos.
Vamos adicionar o resto dos comandos e fechar o bloco:
mcmc ngen=1000000 nruns=2 nchains=4 samplefreq=1000 printfreq=1000 relburnin=yes burninfrac=0.25;
sump;
sumt;
end;
Isso indica que vamos rodar duas corridas com quatro correntes cada uma de 1.000.000 de gerações, amostrando e printando na tela a cada 1.000 gerações, descartando os primeiros 25% das amostras como burnin. Este descarte ocorre porque no começo a busca pode estar em um lugar muito distante no espaço amostral da nossa resposta ótima, o que pode atrapalhar nosso resultado final. Então é melhor esperar as corridas se estabilizarem para começar a considerar os resultados.
Agora salve este novo arquivo como hylodidae_mb.nex.
Uso em servidor de alta performance (plataforma CIPRES)
Agora vá até o CIPRES Science Gateway e siga os passos:
1) Após logar novamente na sua conta, vá até a pasta que você criou anteriormente, clique em Data e então em Upload Data. Depois clique novamente em Escolher arquivos, faça o upload do arquivo hylodidae_mb.nex e clique em save.
2) Agora clique em Tasks e então em Create New Task. Vá em Select Input Data e selecione hylodidae_cipres.nex, depois clique em select data.
3) Vá em Select Tool e seleciuone a opção MrBayes on XSEDE (3.2.7a). Depois clique em 149 parameters set.
4) Como você fez um Data Block do MrBayes, toda a informação da sua análise está lá. Então clique em My Data Contains a MrBayes Data Block (CHECK THIS OR MrBayes BLOCK ENTRIES WILL BE OVERWRITTEN!!!). Clique em Save Parameters e depois em ok.
5) Uma caixa de avisos avisando que o MrBayes utilizado juntamente com o BEAGLE pode ocasionar topologias estranhas. Clique em ok. O BEAGLE é um programa que ajuda muito na velocidade das análises, visto que otimiza o uso dos processadores.
6) Clique em Save and Run Task, depois em OK e espere a análise terminar. Você pode acompanhar o andamento da sua análise clicando em View Status, depois em Intermediate Results e baixando o arquivo stdout.txt.
Agora é só esperar alguns minutos até sua análise terminar.
Depois da análise terminada, baixe os arquivos stdout.txt e infile.nex.con.tre. Abra o primeiro arquivo em um editor de texto e analise a convergência das corridas. Suas análises convergiram? Todos os parâmetros foram amostrados suficientemente? Como saberei isso my god in the sky? Simples, cheque os parâmetros:
1) Average standard deviation of split frequencies:
Deve ser menor que 0.01, você o encontra logo depois que suas corridas terminam:
996000 -- (-14928.552) [...7 remote chains...] -- 0:00:01
997000 -- [-14928.628] [...7 remote chains...] -- 0:00:01
998000 -- (-14937.695) [...7 remote chains...] -- 0:00:00
999000 -- (-14940.569) [...7 remote chains...] -- 0:00:00
1000000 -- (-14929.107) [...7 remote chains...] -- 0:00:00
Average standard deviation of split frequencies: 0.017863
2) ESS = Estimated Sample Size
É bom que seja maior que 100 (ideal maior que 200). Se for menor que 100, pode ser que o parâmetro em questão tenha sido subamostrado. Se encontra na mesma tabela que o próximo parâmetro.
3) PSRF = Potential Scale Reduction Factor; (Gelman and Rubin, 1992)
Deve estar próximo de 1.0 para considerarmos que as corridas convergiram.
95% HPD Interval
--------------------
Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+
----------------------------------------------------------------------------------------------------
TL{all} 4.284699 2.812546 2.621034 7.121098 3.759626 7.97 27.06 1.063
r(A<->C){1} 0.057054 0.000073 0.040775 0.073237 0.056890 290.74 306.58 1.000
r(A<->G){1} 0.166862 0.000197 0.141077 0.194926 0.166592 247.48 252.19 1.001
r(A<->T){1} 0.125490 0.000091 0.105793 0.143947 0.125154 290.35 314.03 1.003
r(C<->G){1} 0.009580 0.000018 0.001579 0.017654 0.009028 350.53 353.75 1.000
r(C<->T){1} 0.635819 0.000390 0.597431 0.674554 0.636070 215.80 234.24 0.999
r(G<->T){1} 0.005194 0.000008 0.000520 0.010899 0.004783 420.00 481.83 0.999
r(A<->C){2} 0.012057 0.000108 0.000021 0.031771 0.009424 217.07 246.52 0.999
r(A<->G){2} 0.114599 0.005139 0.021438 0.275779 0.094842 25.26 33.72 1.001
r(A<->T){2} 0.038292 0.000643 0.001926 0.087802 0.033590 161.89 178.66 1.000
r(C<->G){2} 0.008642 0.000056 0.000024 0.023287 0.006658 311.73 317.28 1.005
r(C<->T){2} 0.812079 0.006490 0.637748 0.935966 0.829227 24.05 36.10 1.001
r(G<->T){2} 0.014331 0.000200 0.000007 0.042335 0.010357 204.21 228.43 1.001
r(A<->C){4} 0.020367 0.000076 0.004103 0.036954 0.019957 144.12 151.00 1.004
r(A<->G){4} 0.598924 0.002802 0.497644 0.691483 0.601599 93.79 107.19 0.999
r(A<->T){4} 0.024198 0.000105 0.003628 0.043450 0.024110 114.11 126.17 1.001
r(C<->G){4} 0.026819 0.000282 0.000042 0.057526 0.025371 123.64 147.11 1.003
r(C<->T){4} 0.298074 0.002280 0.212249 0.387770 0.295780 98.61 110.70 0.999
r(G<->T){4} 0.031618 0.000308 0.000820 0.063361 0.030612 120.24 132.06 1.002
pi(A){1} 0.338588 0.000076 0.321385 0.354943 0.338718 369.98 378.76 0.999
pi(C){1} 0.204448 0.000041 0.192339 0.217906 0.204371 235.33 272.12 0.999
pi(G){1} 0.173476 0.000049 0.161112 0.188153 0.173293 266.98 314.71 1.001
pi(T){1} 0.283488 0.000056 0.269385 0.298957 0.283124 316.56 359.51 1.003
pi(A){2} 0.255659 0.000883 0.197584 0.312548 0.254501 382.77 442.23 1.000
pi(C){2} 0.289313 0.000717 0.236933 0.339975 0.288143 489.60 548.31 1.001
pi(G){2} 0.287198 0.000841 0.232096 0.342260 0.286536 346.39 483.71 0.999
pi(T){2} 0.167831 0.000424 0.128672 0.208453 0.167015 385.21 489.84 1.001
pi(A){3} 0.157894 0.000537 0.116280 0.201765 0.157023 582.17 597.84 1.002
pi(C){3} 0.272938 0.000811 0.220323 0.329828 0.272028 541.59 555.19 1.000
pi(G){3} 0.154086 0.000528 0.112608 0.202821 0.153744 560.43 619.56 1.000
pi(T){3} 0.415081 0.001013 0.350179 0.472114 0.415561 564.27 601.30 1.001
pi(A){4} 0.270619 0.000316 0.234162 0.304620 0.269876 159.65 233.50 1.000
pi(C){4} 0.306865 0.000228 0.277909 0.336675 0.306869 174.98 225.44 1.001
pi(G){4} 0.103127 0.000078 0.086147 0.120477 0.102850 225.04 226.96 0.999
pi(T){4} 0.319390 0.000228 0.288826 0.346058 0.319140 256.89 291.50 0.999
alpha{1} 0.675621 0.021976 0.387983 0.953892 0.668737 172.25 181.58 1.002
alpha{4} 1.819309 0.154146 1.150099 2.602737 1.762954 154.44 180.90 1.000
pinvar{1} 0.446136 0.002591 0.345243 0.540575 0.451033 168.70 174.06 1.001
pinvar{2} 0.793781 0.001839 0.705534 0.867424 0.796941 99.66 105.17 0.999
pinvar{3} 0.842776 0.082729 0.131782 0.999974 0.995003 8.09 8.69 1.067
m{1} 0.504323 0.014953 0.256057 0.694466 0.533102 5.49 10.16 1.075
m{2} 0.187021 0.003785 0.064636 0.295925 0.181816 8.38 17.65 1.050
m{3} 2.304461 6.212413 0.000013 7.380262 1.496332 5.35 8.29 1.087
m{4} 5.688319 1.771090 2.767823 7.589402 5.985662 6.35 9.62 1.079
----------------------------------------------------------------------------------------------------
* Convergence diagnostic (ESS = Estimated Sample Size); min and avg values
correspond to minimal and average ESS among runs.
ESS value below 100 may indicate that the parameter is undersampled.
+ Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman
and Rubin, 1992) should approach 1.0 as runs converge.
4) Podemos checar a amostragem dos parâmetros nas corridas, e o ideal é que pareça aleatório, e que as corridas não pareçam correlacionadas.
Overlay plot for both runs:
(1 = Run number 1; 2 = Run number 2; * = Both runs)
+------------------------------------------------------------+ -14927.49
| 1 2 |
| 2 2 1 |
| 2 1 *1 1 |
| 1 1 1 1 2 1 2 2 2 |
| 21 2 2 2 2 2 2 |
| 2 111 *11 2 21 1 1 1 1 1 * 2 |
| 2 2121 2 2 2 2 1 1 1 2 1 1 1 |
| 2 2 1 1 1 12* 1 1 1 121 1 |
|* 1 1 1 1 2 1 22 22*11 2 2 22 21 21|
| 12 2 2 2 2 2 |
| 2 1 2 1 2|
| 2 1 2 |
| 2 |
| |
| 1 |
+------+-----+-----+-----+-----+-----+-----+-----+-----+-----+ -14941.08
^ ^
250000 1000000
E aí, o que vocês acham? Caso a corrida de vocês não tenha convergido muito bem, podem fazer um teste aumentando o número de gerações. Lembre-se semre de aumentar
a frequência de amostragem dos parâmetros à medida que aumenta o número de gerações. Essa razão deve ser sempre entre 1000 e 10000. Sugiro que testem em uma nova
análise ngen=10000000, samplefreq=1000 e printfreq=1000. Os mais curiosos podem testar também manter o número de gerações e diminuir a frequência
de amostragem de parâmetros para 100, fazendo ngen/samplefreq = 10000. O que mudou nas outras análises?
Abra o arquivo infile.nex.con.tre com o FigTree. Note que há algumas politomias. Isso ocorre porque esta árvore é uma árvore de consenso de maioria, onde clados com menos de 0.5 (ou 50%) de probabilidade posterior são colapsados.
É isso, pessoal! Espero que tenham gostado. Qualquer coisa estou à disposição!
Obrigado!!!
Referências
Gelman A, Rubin DB. (1992). Inference from iterative simulation using multiple sequences. Statistical Sciences 7:457–472.
Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck, JP. (2012). MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61:539-542.