MrBayes: Bayesian Inference of Phylogeny
Dentre os softwares existentes para se fazer análises de inferência Bayesiana, um dos mais utilizados é o MrBayes 3.2 (Ronquist et al., 2012), então vamos analisar a nossa matriz nesse programa. Ele usa arquivos do tipo nexus e não possui interface gráfica. A primeira coisa que devemos fazer é abrir o arquivo hylodidae.nex em um editor de texto (bloco de notas, notepad, etc). Ele será mais ou menos assim:
#NEXUS
BEGIN DATA;
DIMENSIONS NTAX=24 NCHAR=3187;
FORMAT DATATYPE=DNA GAP=- MISSING=? ;
MATRIX
Limnomedusa_macroglossa ---TGGTCCTAGCCTTGAAGTCAGTTTTTACTCAATATATACATGCAAGTCTCTGCACCCCTGTGAGAACGCCCTTAAA-TCCCCGCTAGGGATAA-GGAGCCGGTATCAGGCGCAATAATCTGCCCAAGACACCTAGCTATGCCACACCCCCAAGGGAATTCAGCAGTAATTAACATTGAATATGAGCGCCAGCTTGATTCAGTTAGAGTAAACAGGGCCGGCTAATCTGGTGCCAGCCGCCGCGGTTACACCACGTGGCCCAAATTGACACTTTTCGGCGTAAAGCGTGATTAAAGTCATCCT--ATATTGAAGATTAATTTAAACTAAGCTGTGACACGCTTGTTATCAAGAAACTCAAACACGAAAGTTGCTTCAACCCTACCAACTTGAACTCACGACAGCTGGGGTACAAACTGGGATTAGATACCCCACTATGCCCAGCCGTAAACTTTAACCTACACCAC-CATCGCCTGGGAACTACGAGCAAAGCTTAAAACCCAAAGGACTTGACGGTACCCCACATCCACCTAGAGGAGCCTGTCCTGTAATCGATAACCCCCGTTAAACCTCACCATTTTTAGCTTATCAGCCTGTATACCTCCGTCGCCAGCTTACCACGTGAGCGAGATTTAGTGAGCTTAATGTCCATACGTCAGTACGTCAGGTCAAGGTGCAGCAAATAAAATGGGAAGAGATGGGCTACACTCTCTATAATAGAAGAAACGAAAGACTAATTATGAAA-CCTAGTCAGAAGGCGGATTTAGTAGTAAAGGGAAACAAGAGTGTTCACTTTAACTTGGCCCTGGGGTGTGTACACACCGCCCGTCACCCTCTTCA-AAGGTAATAGACAGTATTTAACAAGTTTTAG-CTTTTCAGAAGAGGCAAGTCGTAACATGGTAAGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCTTAA-CAAAAGCATCTCGCTTACACCGAGAAGTCATCTGTTAAAACCAGATTATTTTGAGCTAAAAATCTAG-CCCAACCTAAGCATATGAAAAAATAAAAATT-TTTTTACACTAATCAAAACATTCTTATTTCCTAGTAAAGGCGATTGAAAAGTCTAATTGGAGCTATATACAATGTACCGCAAGGGAAAGATGAAATAATAATGAAATAC--TCTCAAGCACAATTCAGTAGAGATTCAACCTCGTACCTTTTGCATCATGGTTTAACTAGTCTAATCAAGCAAAACGA-ACTTTAGTTTGACCTCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTATTGAGCCGACCCGTCTCTGTTGCAAAAGAGTGGGAAGACCTTCAAGTAGGGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTGCTCAAGAAAAGGATTTAAGTTCAGCCTAAAATTACCTCAA----TTTCACGAAATAAGGTCTTAATTTTAGAGTTATTCAAATAAGGTACAGCCTATTTGAAACAGGGTACAACCTAGATCAGCGGGTAATCTCTTTATA--TAATCTAACCAAGTAGGCCTAAAAGCAGCCACCTTTAA-AATAGCGTCACAGCTTAATTAAAT-GATTGATAAATCTCATTAAAATTTTAGAACCCCTACTCTATATTGAGCAATTCTATAGCC-CTATAGAACATATTATGTTAAAACTAGTAACAAGAAGAAAACCTTCTCTTTAATGTAAGTGTAAATCAGGTAGGATAAACCACTGATAATTAACATCCATGAGCTGAG-AGTATTAACCCATCAAGAAAACTATACTTCTTTATATGTTAACCTAACACAAGAGCATTTCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAATTTTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGATTAACAATAAGAGGTCCAGCCTGCCCAGTGAC-TCAGTTTAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTTTTTCCAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGGATTAAATTATAAGACGAGAAGACCCTATGGAGCTTTAAACAAAGTAACAATTGCTACTCTACTTTC-TAATTTCTGAAAACTAAC--TTATTTTAGCCTTATGA-TTACTAGTTTTAGGTTGGGGTGACCGCGGAGAATAATTTAACCTCCACATTGAATGAATT--CTATTCTAAGCTAAAAGCCACGACTTTAAGCATCAATAAATTGACATCTATTGATCCAATACTTTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTCAAGAGCTCCTATCGACAAGTGGGTTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT----------------------------------------------------------------------AACTCTCTATCTCATCTTCGGTGCCTGAGCCGGAATAGTGGGGACCGCCCTTAGTCTTCTTATCCGGGCTGAGCTAAGTCAGCCCGGCTCACTTCTAGGGGATGACCAAATTTACAATGTAATCGTAACCGCCCATGCCTTTGTTATAATCTTCTTCATGGTGATGCCAATTATAATTGGGGGTTTCGGAAACTGGCTCGTTCCTTTAATGATCGGGGCCCCAGACATGGCGTTCCCCCGAATGAACAATATAAGCTTCTGACTTCTCCCTCCCTCTTTCCTCTTACTTTTGGCCTCTGCAGGAGTGGAAGCCGGGGCCGGAACCGGGTGAACCGTTTACCCCCCTTTAGCAGGAAATCTAGCTCACGCCGGACCCTCCGTGGATTTAACTATTTTTTCTCTCCACCTAGCAGGTGTCTCTTCGATCCTGGGAGCAATTAACTTTATTACTACCATCCTAAACATAAAACCTCCCTCTATAACTCAATACCAAACGCCACTGTTTGTTTGGTCCGTATTAATTACTGCTGTTCTCCTCCTACTTTCACTCCCAGTCCTAGCCGCGGGCATTACTATACTTCTCACTGATCGTAACTTAAATACAACATTTTTTGACCCGGCAGGAGGGGGGGACCCAATCCTATACCAACACCTATTT-------
Crossodactylus_aeneus ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCGCCTGTTTATCAAAAACATCGCCTCTTGTTTATTTATAAGAGGTCCAGCCTGCCCAGTGAC-TCAATTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTTGTATGAATGGCATCACGAGGGTTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATAAACCTATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATAGCAATTGCTTATTT-TATAC-TATATCCCGATATTTAATACTTAATTTAGCATTATGAACTACAAATTTTAGGTTGGGGTGACCATGGAGCAAAAATCAACCTCCACATTGAATAAAACTTTACTTTTTAGCAAAAAGTTACCACTTTAAGCACCAACATATTGACATTAATTGACCCAATA--ATTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTAGGTTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAGTTCAGACCGGA????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Crossodactylus_bokermanni G------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAATAAGAGGTCTAGCCTGGCCAGTGAC-CTAGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTTTCTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAACATATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATAACAAGTGCTTCTTT-CTATCTCACATCCTGATGAATAATTTTTAATCTAGCATTTTAACTTATAGATTTTAGGTTGGGGTGACCATGGAGCAAAAACTAACCTCCATATTGAAAAACCT--CATTTTTAAGCAAAAAGTTACTACTTTAAGCATCAACATATTGACATTAATTGACCCAATA--ATTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTAGGTTTACGACCTCGATGTTGGATCAGGGTGTCCTAGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCT--------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Crossodactylus_caramaschii ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGAGGTCCTGCCTGCCCAGTGAC-CTAGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTCTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAATATATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATAACAAATGCTTATTT-TTTACTCTCATCCAGATGACTGACTATTATTTTAGCATTTTGACCTATAAATTTTAGGTTGGGGTGACCATGGAGCAAAAATTAACCTCCATATTGGAAAAACCT-TATTTTTAAGCAAAAAGTTACCACTTTAAGCATCAACACACTGACATTAATTGACCCAATA--GTTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTAGGCTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAAC--------------------------GTGATAATTACCCGATGATTTTTTTCTACCAACCACAAAGATATTGGAACCTTATACTTAGTATTTGGCGCATGAGCTGGAATGGTTGGGACCGCCCTCAGCCTGCTCATTCGAGCTGAATTAAGCCAGCCAGGCTCCCTGCTAGGCGATGACCAGATTTATAATGTTATTGTTACTGCACATGCTTTCGTAATAATTTTTTTTATAGTGATGCCGATTATAATCGGCGGATTCGGTAACTGACTAGTACCACTTATGATTGGGGCCCCTGATATAGCATTCCCCCGTATAAATAATATGAGCTTTTGACTCCTCCCCCCCTCATTTCTTCTTCTTTTGGCTTCCGCTGGGGTAGAGGCTGGGGCCGGAACCGGTTGAACTGTCTACCCCCCACTCGCCGGAAATATGGCCCATGCCGGACCATCCGTAGACTTAACAATTTTTTCTCTCCATCTTGCTGGAGTTTCCTCAATCTTAGGCGCAATTAACTTTATTACCACAATTCTAAATATAAAACCCCCCTCTGTTACCCAGTACCAGACCCCCTTATTTGTGTGGTCTGTCCTGATCACAGCTGTTCTTCTTCTCCTTTCCCTGCCTGTTTTAGCCGCCGGAATCACCATACTTCTCACGGACCGCAACCTAAATACTACATTTTTTGACCCGGCAGGTGGAGGAGACCCAA---------------------------
Crossodactylus_schmidti ---TGGTCCTAACCTTG-AATCAATTTTTACTGTACATACACATGCAAGTCTCCGGACCCCCGTGAAAACGCCCTTAAACTTCCTGCTAGGAACAAGGGAGCTGGTATCAGGCACAAC-TTCTGCCCAAAACACCTAGCTACGCCACACCCACAAGGGAATTCAGCAGTGATTAACATTGAATATTAGCGTCAGCTTGATTCAGTTAAAGTAAATAGAGCCGGCCAATCTGGTGCCAGCCGCCGCGGTTACACCACGTGGCTCAAATTGATTTTTACCGGCGTAAAGCGTGATTAAAGTCAACCCC-CTATTGAAGTCAAATTTAAACTAAGCCGTGACACGCTTGTTCTTTAGAAGCACAAAAACGAAAGTTACTTCATCTTAACCTACTTGAATACACGACAGCTTGAGCACAAACTGGGATTAGATACCCCACTATGTCCAGCCGTAAACTTTAAATTACTCCTTATACCGCCAGGGAACTACGAGCTAAGCTTAAAACCCAAAGGACTTGACGGTACCCCATGTCCCTCTAGAGGAGCCTGTCCTATAATCGATAACCCCCGTTTAACCTCACCATTTTTGGCTAATCAGTTTGTATACCTCCGTCGCCAGCTTACCCCGTGAGCGAATCTTAGTGAGCCTAACGCCCATACGCCAATACGTCAGGTCAAGGTGCAGCAAATAAAATGGAAAGAGATGGGCTACACTTTCTAATATAGAAAATACGAAAAACTACCTATGAAATCCTAGTTTGAAGGCGGATTTAGTAGTAAAAAGAAACCAGAGAGTTCTTTTTAACAAGGCCCTGGGGTGTGTACACACCGCCCGTCACCCTCTTCA-AAGCCAAATACCAGTTATTAACAAAATCTG--CTTAACAGAAGAGGCAAGTCGTAACATGGTAAGCATACCGGAAGGTGTGCTTGGAATCAAAGTATAGCTTAATCTAAAGCGCCTCGCTTACACCGAGCACATATTTGTTAAAATCAAATTACTTTGAGCTGAAAATCTAG-CCCCACATCTTCTTATGATCAAC-TTACACC-TAACTTTTTCCACTAAAACATTTTATTATTTAAGTAAAGGCGATCGAAAAAT-TAACAAGAGCGATAAATATTGTACCGTAAGGGAAAGATGAAATAATA-TGAAACA---TTTTAAGCACAAAAAAGTAGAGATTTAATCTCGTACCTTTTGCATCATGATTTAACTAGTTTACCCAGGCAAAACGA-ATTTAAGTTTGGCCCCCCGAAACTAAGCGAGCTACTTCAAGGCAGTTTTATGAACTCATCCGTCTCTGTTGCAAAAGAGTGGAAAGACCTTCAAGTAGGGGTGACAGACCTAACGAGCCTAGTGATAGCTGGTTACTCAAGAAAAGGATTTTAGTCCTCCCTAACGTCTATTAGA----TATCACAAAATAAAATTTAGACTTTAGAGTTATTCAATTAAGGTACAGCCTATTTGAATCAGGATACAACCTCAACTAATGGGTAAGCTCAATTT---TCTTGTATCTAAGTGGGCCTAAGAGCAGCCACCTATAA-AATAGCGTCAAAGCTTAACTACAT-TTTTCTTTAATACCTATATTTATTACCAACCCTTTACTTGTAATGAGTACTTCCATATTTTTTATGGAACTTATTATGTTAAAACTAGTAACAAGAATTAGATTTTCTCTTTAATGCAAGTGTATATCAGAAAGGACACTCCACTGACTATTAACATCCATGAATTTAG-GGTAGTAACCAAACAAGAAAACTCTACCTTCCCCATTGTTAATCTAACACAAGAGCATTTCAAGAAAGATTAAAAGGAAAAGAAGGAACTCGGCAAATATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGCATCTCTATAAGAGGTCCAGCCTGCCCAGTGAC-CCAGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCACCACGAGGGTTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTTTATTATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATGACAAATGCTTCTTT-TTTAC-TACATCCAGATAATTAATTATTAATTTAGCATTTTGACCTATAAATTTTAGGTTGGGGTGACCATGGAGCAAAAGTTAACCTCCACATTGAAAAAACCT-CATTTTTAAGCTAAAAGTTACCGCTTTAAGCATCAATATATTGACATTAATTGACCC-ATA--ATTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTGGGTTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT----------------------------------------------------------------------AACCCTGTACTTAGTGTTTGGGGCATGAGCCGGAATGGTTGGAACCGCCCTTAGTTTACTCATTCGAGCCGAATTAAGCCAACCAGGTTCTCTGCTAGGAGACGACCAGATTTATAATGTTATCGTAACTGCCCATGCCTTTGTAATGATTTTTTTTATGGTTATACCCATTATAATTGGGGGATTTGGCAACTGATTAGTTCCTCTAATAATTGGAGCCCCTGATATAGCATTCCCTCGTATAAACAATATAAGCTTTTGACTTCTGCCCCCCTCATTTCTTCTTCTCCTAGCCTCCGCCGGAGTGGAAGCTGGGGCTGGAACTGGCTGAACTGTCTACCCCCCTCTAGCCGGAAATATGGCCCATGCCGGACCGTCCGTAGACTTAACAATCTTTTCCCTTCATCTTGCTGGAGTCTCATCAATCTTAGGTGCGATCAACTTTATTACTACGATTTTAAATATGAAACCCCCTTCTGTGACCCAATACCAAACCCCCTTATTTGTCTGATCCGTTCTTATTACAGCTGTCCTTTTACTTCTTTCTTTACCTGTCTTGGCCGCCGGGATTACTATACTCCTCACAGACCGCAATTTAAATACTACCTTTTTTGACCCGGCAGGCGGGGGAGATCCCGTCCTGTACCAACATTTATTC-------
Crossodactylus_werneri ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGTGAC-CCAGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTCTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAAAATATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ATATAACAAATGCTTCTTTATCTACACACATTCAGATGACCAACTACTAATTCAGCATTTTGACTTATAAATTTTAGGTTGGGGTGACCGTGGAGCAAAAATTAACCTCCATATTGAAAAAACC--TCATTTTAAGCAAAAAGTTACCACTTTAAGCATCATTACACTGACATTAATTGACCCAATA--ATTTGATCAATGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCTACTTCAAGAGCTCTTATCGACAAGTAGGTTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAA---------------------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_amnicola --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAGCCCTTCGCTTACACCGAAAAATTATCTGTTAAACCCAGATTATTTTGAGCTAAAAATCTAACCCCCACCNTATATAATGTCATCT-CAAAANTCTTAATCATTNAATTAAAACATTCTTATAATTTAGTAGAGGCGATCAAAAAAT-TAATTGGAGTAATAAATATTGTACCGCAAGGGAAAGATGAAATAAAAATGAAAAAT--TATNAAGCACAAAAAAGTAAAGATTTACCCTTGTACCTTTTGCATCATGATTTAACTAGCTTACCCCAGCAAAACGA-CATTAAGTCGGACCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCCCATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGCGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTACTCAAGAAAAGGATTTTAGTCCCGCCTAAAGCAAACACTATTA-CTTTAAAATAAAAATTTNCGGCTTTAGAGCAATTCAATTAAGGTACAGCCTATTTGAAACAGGATACAACCTCGACTAACGGGTAACTATTACCTA--CTTCATTCTTAAGTGGGCCTAAAAGCAGCCACCTTTAATAATAGCGTCAAAGCTTTACTATGC-AAATATTTAATACCAATTTTTCTTCATAACCCTTTATTAGTACTGAGTAAATCTATATAA-CTATAGAAACTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TATATGCAGGTGTACATCAGAAAGGACATACCACTGATAATTATCACTCATGAACCCAA-AGAAGCAACTTAGCTAGAAAATTCTTCTACACTTAATGTTAATCTAACACAAGCGCATATTAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACATAAGCCTCGCCTGTTTACCAAAAACATCGCCTCTTGC--AAATTTAAGAGGTCCAGCCTGCCCAGTGAC-TCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGCTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAGCTTATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAATAACACCTGCTAATCCTCTTAC-CATATTCAGATACTCTATTTATACCTTAGCACCATGA-TTATAAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAACTAACCTCCACACTGAAGGAACT---CTTTCTAAGCAAAAAGCTACAACTTTAAGCATCAACAAATTGACACTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAAC----------------------------------------------------------------------------------AACCCTATATCTTGTATTTGGAGCATGAGCTGGGATAGTGGGAACTGCCCTAAGCCTACTCATCCGAGCCGAACTGAGCCAACCTGGCTCTCTCTTAGGTGACGACCAAATCTACAATGTTATCGTTACCGCCCACGCTTTCGTTATAATCTTTTTTATAGTAATGCCCATTATGATCGGGGGGTTCGGTAATTGACTGGTCCCCCTAATAATCGGAGCCCCGGATATAGCCTTTCCACGGATAAATAACATAAGCTTTTGACTCCTTCCCCCATCCTTTCTTCTTCTTTTAGCTTCCGCCGGAGTTGAGGCAGGCGCAGGAACAGGGTGAACAGTCTATCCCCCTCTTGCCAGCAACTTAGCCCATGCCGGACCCTCTGTAGATCTTACCATTTTTTCTCTTCACCTTGCCGGTGTTTCATCTATCCTTGGCGCCATTAACTTCATCACCACAATCCTAAATATGAAACCCCCATCTGTAACCCAGTATCAAACCCCCCTTTTTGTGTGATCTGTCTTAATTACTGCTGTTCTCCTTCTCTTATCCCTTCCGGTCTTAGCCGCTGGTATCACGATGCTTTTAACAGATCGAAATTTAAATACAACATTTTTTGACCCGGCAGGTGGGGGAGACCCCATTCTATATCAACACTTATTTTGAT---
Hylodes_asper ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAATGGCATCACGAGGGTTGAACTGTCTCCTTTCTCTAATCAGTGAAACTAATTTTCCCGTGAAGAAGCGGGAATAATTCTATAAGACGAGAAGACCCTATGGAGCTTTAAAT-ACATAACACTTGCTAT--TACCTAT-AATCTCCCGATATTCAATCTTTATCCAAGCATNATGA-TTATTAATTTTAGGTTGGGGCGACCACGGAGCAAAAATTAACCTCCACATTGTAAAAATT----TTTTTAAGCAAAAAGTTACAACTTTAAGCATCAATAAATTGACATTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGG----------------------------------------------------------------------------------------------------------------------------------CCACAAACCACAAAGATATCGGAACCCTATATCTTGTTTTCGGGGCATGAGCCGGAATAGTCGGAACAGCCCTTAGCCTCCTAATTCGAGCTGAATTAAGCCAACCCGGATCCCTCTTAGGAGACGACCAAATTTATAATGTGATTGTTACCGCACATGCCTTTGTAATAATTTTTTTTATGGTCATACCAATTATAATTGGAGGATTTGGAAACTGACTAGTACCTTTAATAATTGGGGCCCCCGATATGGCTTTTCCTCGGATAAACAATATGAGCTTTTGATTGCTCCCACCATCTTTTCTTCTTCTTCTAGCCTCTGCCGGAGTAGAAGCAGGTGCAGGAACGGGCTGAACAGTTTACCCCCCTCTAGCTAGCAATTTAGCCCATGCAGGGCCCTCCGTTGACCTAACTATTTTCTCTCTTCATTTAGCTGGTGTCTCCTCTATTCTTGGTGCCATTAATTTCATCACAACTATTTTGAATATGAAGCCCCCNTCTATAACTCAGTATCAAACCCCACTCTTTGTTTGGTCCGTCCTTATTACCGCTGTTCTTCTCCTTCTCTCTCTCCCAGTTCTAGCCGCTGGAATCACTATGCTTCTCACTGATCGAAATTTAAACAC--------------------------------------------------------------
Hylodes_caete ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GCCCTTCGCTTACACCGAAAATTTATCTGTTAAACTCAGATTATTTTGAGCTAAAAATCTAA-CCCCACCTTATTTTATGCAACCC-TAAAACT-TTAATCATCTAATTAAAACATTTTTACAGTTTAGTAGAGGCGATCAAAAAAT-TAATTGGAGTAATAAATA-TGTACCGCAAGGGAAGGATGAAATAAAAATGAAA-----ACTTAAGCACAAAAAAGTAAAGATAAACTCTTGTACCTTTTGCATCATGATTTAACTAGTCTACCCCAGCAAAACGA-CTTTAAGTCAGGCCCCCCGAAACTAAGTGAGCTACTTCAAGACAGCTTAATGAGCACATCCGTCTCTGTTGCAAAAGAGTGGAGAGATCTTCAAGTAGAGGTGACAGACCAAACGAACTAAGTGATAGCTGGTTACTCGAGAAAAGGACCTCAGTCCCACCTAAAGTTAGCAATATTA-ATTTTAAACAATAATTTTCGGCTTCAGAGTAATTCAATTAAGGTACAGCCTATTTGAAACAGGGTACAACCTAAACTAACGGGTAATT-TGATATA--CCTAATTTTTAAGTGGGCCTAAAAGCAGCCACCTTTTA-AATAGCGTCAAAGCTTTATTATAT-TTGTATTTAATACCGAATCTGTTATTTAACCCTCTATTAGTATCGAGTAAATCTATATGC-TTATAGAAATTTTTATGTTAAAACTAGTAACAAGAAATAGATTTCCTC-TGTATGTAAGTGTATATCAGAAAGGACATACCACTGATCATTAACATTCATGAGCCCAA-AGCAGTAACTTAACTAGAAAACTCTTCTATATAAAATGTTAACCTAACACGAGCACATATCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACACTAACCCCGCCTGTTTACCAAAAACATCGCCTCTTGTA-AAATTTAAGAGGTCCAGCCTGCCCAGTGAC-CCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTATTATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAACAACAATTGCTAACCTATTTAC-AATACTTAGGTATCTAATCTAAATCCTAGCATTATGA-TTTTAAGTTTTTGGTTGGGGTGACCACGGAGTAAAAATTAACCTCCATATTGGAAGAATC--TCTTTCTAAGCAAAAAGTTACTACTTTAAGCATCAACAAATTGACATCCATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCATTTTAAGAGCTCTTATCGACAAATGGGTTTACGACCTCGATGTTGGATCAGGGTACCCCCGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAGT----------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_charadranaetes ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TATCAAAAACATCGCCTCTTGTA-AAATCTAAGAGGTCTAGCCTGCCCAGTGACTTTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTGTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTATTATATAAGACGAGAAGACCCTATGGAGCTTCAAAC-AAATTACATTTGCTATT-TTTCTAC-AATCTTCCGATATTTAAATTTTATCCTAGCATTATGT-CTATTAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCATATTGAAAGATTT---ATTTCTAAGCAAAAAGTTACAACTTTAAGCATCAATAAATTGACATTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCATTTTAAGAGCTCTTATCGACAAATGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAG-----------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_fredi -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCTTAA-CTAAAGCCTTTCGCTTACACCGAAAAATTATCTGTTAAACCCAGATTATTTTGAGCTAAAAATATAA-CCCCACTTTCTTTTATATCCTT--ACAAATT-TAAATTTTAAAATTAAAACATTCTCAAAGTCAAGTAGAGGCGATCAAAAAAT-TAATTGGAGTAATAAACAATGTACCGCAAGGGAAAGATGAAATAAAA-TGAAATAA--TATCAAGCACAAAAAAGTAAAGATTAACTCTTGTACCTTTTGCATCATGATTTAACTAGTATACCCCGGCGAAACGATTTTTAAGTCGGACCCCCCGAAACTAAGTGAGCTACTTCAGGGCAGCTTTTTGAGCATATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGGGGTGATAAACCAAACGAACTAAGTGATAGCTGGTTACTCGAGAAAAGGATCTTAGTCCCGTCCAAAGCAAACATTATTA-ATTAAAAATAAAAATTTTTAGCTTTGAAGTAATTCAATTAAGGTACAGCCTACTTGATACAGGAGACAACCTAAACTAATGGGTAATTTAAATACA---TCTTTTTTCAAGTAGGCCTAAAAGCAGCCACCTTTAA-AATAGCGTCAAAGCTTCACTACAT-ACGTATTTAATACCAAACTCATCACTTAACCCTTTATTAGTATCGAGTAGATCTATATAG-TTATAGAAATTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TATATATGAGTGTACACCAGGAAGGACACACCACTGACCATTAACATCTTTGAACCTAA-AGCAGCAACTT-ACAAGAAAATTCTTCTTTACCAAATGTTGATCTAACACAAGCATATATCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACACTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTA-AAATTTAAGAGGTCTAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTCTTTCTAATCAGTGAAACTAATCTTCCTGTGAAGAAGCGGGAATTATTATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAACAACACTTGCTAATTTATTTGT-GATACTCAGGTCACCAACTTTAAACCTAGCATTATGATTTTTAAGTTTTTGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCATGCTGAAAGAACT----TCTCTAAGCAAAAAGTTAATACTTTAAGCATCAAAAAATTGACATTCATTGACCCAATA--TTTTGATTAACGAACCCAAGTTACCCTAGGGATAACCAGGGCCAATTC------------------------------------------------------------------------------------------------------------------------------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_lateristrigatus ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CAAAAACATCGCCTCTTGT--AAATTTAAGAGGTCTAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTACACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAACATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAGTAGCACTTGCTAAT-TAATTAT-TATACTCAGGTATCTAACTCTTATCCTAGCACTACTGCCTACAAGTTTTCGGTTGGGGTGACCGCGGAGTAAAAACCAACCTCCACACTGAAAGAACT---CTTTCTAAGCAAAAATCTACAACTTTAAGCATCAATAAATTGACATCTATTGACCCAATA--CTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTAAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGCAGCCGCTACTGAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGA------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_meridionalis -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCTTAA-TTAAAGCCTTTCGCTTACACCGAAAAACTATCTGTTAAATCCAGATTATTTTGAGCTAAAAATCTAA-CCCCACTTTATGTTATGCTATAT-GTAAACT-TTAATCATTTAATTAAAACATTTTTACAACTTAGTAGAGGCGATCAAAAAAT-TAATTGGAGTTATAAATTATGTACCGCAAGGGAAAGATGAAATAAAAATGAAAA----CATCAAGCACAAAAAAGTAAAGATTAAGTCTTGTACCTTTTGCATCATGGTTTAACTAGCTTACCCTAGCGAAACGA-TTTTAAGTCAGACTCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCATATCCGTCTCTGTTGCAAAAGAGTGGTGGGACCTTCAAGTAGAGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTGCTCGAGAAAAGGATTTTAGTCCCGCCCAAAGCAAACATTATTATTTTTTTAAACAAAATTTTTAGCTTTAGAGTAATTCAATTAAGGTACAGCCTATTTGAAACAGGATACAACCTTGCCTAATGGGTAAA--TAACATG--CTACATCTTTAAGTAGGCCTAAAAGCAGCCACCTTTTA-AAAAGCGTCAAAGCTTCGCTATGT-TAATATTCAATACCAACCTCACAAATTAACCCTTTATCGTCATTGAGCAAATCTATACAT-CTATAGAAACTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TTTATGTAAGCTTAAATCAGAATGGACACACCACTGATAATTAACACCTATGAATCGAA-AGTAGCAACCCAACAAGAAAACTCTTCTGTACTCAATGTTAATCTAACACAAGCACATATTAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAATATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTCGTA-GAATTTGAGAGGTCCAGCCTGCCCAGTGAC-CTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTTTCTCTAATCAGTGAAACTAATCTTTCCGTGAAGAAGCGGGAATTATCCTATAAGACGAGAAGACCCTATGGAGCTTTAAAC-TAATAACACCTGCTAATTTAAATAT--TTATTCTGATTTTTAACCTATATTCTAGCACAATGA-TTAAGAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCACATTGAATTAGAG--CATATCTAAGCAAAAAACCACATCTTTAAGCATCAACAAACTGACATTTATTGACCCAATA--ATTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTACCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT-----------------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Hylodes_nasus --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAGCCCTTCGCTTACACCGAAAAGTTATCTATT-AATACAGATTATTTTGAGCCAACAATCTAA-CCCCACTTCACCCTATAACATCT-CAACACT-TTTGTGATAAAATCAAAACATTTTAATAACTTAGTAGAGGCGATCAAAAAGT-TAACTGGAGTAATATCAAATGTACCGCAAGGGAAAGATGAAATAAAAATGAAATATC-TTCCAAGCACAATAAAGTAAAGATTCTCTCTTGTACCTTTTGCATCATGATTTAACTAGTCTACCTCAGCAAAATGA-CTTTTAGTCTAGCCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCATATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGAGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTACTCGAGAAAAGGATCTTAGTCCCACCTAAAACTATTG-TATTT-TTATAAAATAAAAATTTATAACTTTAGAGTAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTTTACTAATGGGTAAAC-TTCTACT--CACAATTTTTAAGTGGGCCTAAAAGCAGCCACCTTTCA-AAAAGCGTCAAAGCTTTACTATCG-TGATAAAAAATATCAATTTTGTTACACAACCCTTTACTAGTACCGAGTAATTCTATATTA-CTATAGAAATTTTTATGTTAAAACTAGTAACAAGAATAAAATTTTCTC-TGTATGTAAGTGTACACCAGGAAGGACAAACCACTGGTAATTAACATTCGTGAATCTAATAGAAGCAACTTAACAAGAAAAACCTTCTATAACCCATGTTAATCTAACACAAGCACATGTCAAGAAAGATTAAAAGGAAAAGAAGGAACTCGGCAAACATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTA-AAATTTTAGAGGTCCAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTTTTCCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATAATTTTATAAGACGAGAAGACCCTATGGAGCTTTAAAT-GAATAACACCTGCTAAC-TATCTAC-AATATTCAGATACTTAACTTATATTTTAGCATGATGA-TTACTAGTTTTAGGTTGGGGTGACCACGGAGTAAAAATTAACCTCCATATTGTAAAGAAT---AATTTTAAGCAAGAAGTTACAACTTTAAGCATCAATAAATTGACATTTATTGACCCAATA--TTTTGAGCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAAC------------------------------------------------------------------------------------CCCTTTATCTTGTTTTCGGAGCATGAGCCGGAATAGTCGGAACTGCTCTAAGCCTTCTTATCCGTGCCGAACTAAGCCAACCTGGGTCGCTCTTGGGAGACGACCAAATTTACAATGTGATTGTTACCGCCCATGCTTTCGTGATAATTTTTTTTATAGTTATACCCATTATGATCGGAGGGTTTGGAAACTGACTGGTGCCCCTAATGATTGGAGCCCCCGACATGGCCTTCCCTCGAATAAACAACATAAGTTTCTGACTTCTTCCTCCATCTTTTCTTCTTCTTTTAGCCTCCGCCGGAGTAGAAGCAGGTGCAGGAACAGGGTGAACAGTCTACCCCCCCTTAGCTAGCAATCTCGCTCATGCAGGTCCATCCGTGGATCTAACTATCTTCTCTCTCCACCTAGCTGGTGTCTCATCTATTCTTGGGGCCATTAACTTTATTACCACAATTCTTAACATGAAACCACCCTCAATGACCCAGTATCAGACCCCACTTTTTGTTTGATCTGTCCTTATCACTGCAGTTCTTCTGCTCCTTTCCCTCCCAGTCCTAGCCGCTGGCATCACTATGCTACTCACAGACCGAAATCTAAACACAACATTCTTTGACCCGGCAGGAGGGGGAGATCCTATTCT-----------------------
Hylodes_ornatus --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAGCCCTTCGCTTACACCGAAAATTTATCTGTTAGACCCAGATTATTTTGAGCTAAAGATCTAA-CCCCACCTTATTTTATGTCATCT-CAAGACT-TTAATCATTTAACTAAAACATTCTTATAGTTTAGTAGAGGCGATCAAAAAAT-TAATCGGAGTAATAAATATTGTACCGCAAGGGAAAGATGAAATAAAAATGAAAAAT--TTTTAAGCACAAAAAAGTAAAGATTCACCCTTGTACCTTTTGCATCATGATTTAACTAGCTTACCCCAGCAAAACGA-TATTAAGTCGGACCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCATATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGCGGTGATAGACCTAACGAACTTAGTGATAGCTGGTTACTCAAGAAAAGGATTTTAGTCCCGCCTAAAACAAACATTATTA-TTTTAAAATAAGAATTTTCGGCTTTAGAGCAATTCAACTAAGGTACAGCCTATTTGAAACAGGATACAACCTTGACTAATGGATAAC--TTATATA--CTTCATTTTTAAGTGGGCCTAAAAGCAGCCACCTTTAATAATAGCGTCAAAGCTTTATTATGT-AAATATTTAATACCAATTTTTTTTCATAACCCTTTATTAGTACTGAGTAAATCTATACAA-TTATAGAAACTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TATATGCAGGTGTATATCAGAAAGGACATACCACTGATAATTATCATTCGTGAACCCAA-AGAAGCAACTTAACTAGAAAACTCTTCTATATTTAATGTTAATCTAACACAAGCGCATGTCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACATTAGCCTCGCCTGTTTACCAAAAACATCGCCTCTTGC--AAATTTAAGAGGTCCAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGCTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTAATCTATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAATAACATTTGCTATC-CTTTTAC-AACATTCAGATATCTAATCTTTACCTTAGCACTATGA-TTACAAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCACATTGAAGGAACT---CTTTCTAAGCAAAAAGCTACAACTTTAAGCATCAACAAATTGACACTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCATTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAAC-----------------------------------------------------------------------------------ACTCTATATCTTGTGTTTGGAGCATGAGCTGGGATAGTCGGAACTGCCCTAAGCTTACTCATCCGAGCTGAACTTAGCCAGCCCGGCTCCCTCTTAGGGGACGATCAAATTTACAACGTCATCGTCACCGCACATGCCTTCGTTATAATCTTCTTTATAGTCATGCCCATCATAATCGGCGGGTTCGGTAACTGACTGGTTCCCCTAATAATTGGAGCCCCGGACATGGCCTTTCCCCGAATAAATAATATAAGCTTTTGACTCCTTCCACCATCCTTTCTTCTTCTTTTGGCCTCCGCAGGTGTTGAAGCTGGCGCAGGAACAGGGTGGACAGTCTACCCCCCTCTTGCTAGCAACTTAGCCCACGCCGGACCCTCTGTAGATCTTACTATCTTTTCCCTTCACCTCGCCGGTGTATCGTCCATTCTTGGTGCCATTAATTTTATCACCACGATCCTAAATATGAAGCCCCCATCTGTAACCCAGTATCAAACTCCCCTTTTTGTGTGGTCTGTTTTGATCACTGCTGTTCTTCTTCTATTATCCCTTCCAGTATTAGCCGCTGGCATCACAATGCTTTTGACAGATCGAAATCTAAATACAACATTCTTTGACCCGGCAGGTGGAGGAGACCCCATTCTTTAC-------------------
Hylodes_perere ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????--------------------------------------------------------------------------------------GGGAACTGCCCTAAGCCTGCTCATCCGAGCCGAACTAAGCCAGCCTGGCTCACTCCTAGGGGACGATCAAATTTACAATGTTATCGTTACCGCACACGCTTTTGTTATAATTTTTTTTATAGTAATGCCCATTATGATCGGTGGGTTTGGTAATTGATTGGTCCCCTTAATAATTGGAGCCCCAGATATAGCCTTTCCACGGATAAATAACATGAGCTTTTGACTCCTTCCCCCATCCTTTCTTCTTCTTTTAGCCTCCGCCGGAGTTGAGGCGGGCGCAGGGACAGGGTGGACAGTCTACCCCCCTCTTGCCAGCAACTTAGCCCATGCCGGACCCTCTGTAGATCTAACCATTTTTTCCCTTCACCTTGCCGGTGTGTCATCCATCCTTGGTGCCATTAATTTCATCACTACAATCCTGAACATAAAACCCCCATCTGTAACCCAGTATCAAACCCCCCTTTTTGTGTGATCTGTTTTAATTACTGCTGTTCTCCTTCTCTTATCCCTTCCGGTCTTAGCCGCTGGTATCACAATGCTTTTAACAGATCGAAACTTAAACACAACATTTTTTGACCCGGCAGGTGGGGGGGACCCCATTCTCTAC-------------------
Hylodes_phyllodes -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGTATACCGGAAGGTGTGCTTGGAAACAAAGTATAGCTTAA-CCAAAGCCTTTCGCTTACACCGAAAAACTATCTGTTAAACCCAGATTATTTTGAGCCGAAAACCTAA-CCCCACCACCCCTTTTATATCCCACAAAACC-TTAATTATTCTACTAAAACATTTTCACAGCTTAGTAGAGGCGATCAAAAAAC-TAATTGGAGTAATAACTTTTGTACCGCAAGGGAAAGATGAAATAAAATTAAAA-----CTTAAAGCACAAAAAAGTAAAGATTAACCCTTGTACCTTTTGCATCATGATTTAACTAGTCTTCTTCGGCAAAACGATTTTTAAGTCGATTCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCACACCCGTCTCTGTTGCAAAAGAGTGGAAAGACCTTCAAGTAGAGGTGACAGACCAAACGAACTTAGTGATAGCTGGTTACTCGAGAAAAGGATTTAAGTCCCCCCTAAAGCAAACACTATTA-ACTAAAAATAATAATTT--AGCTTAAGAGCAATTCAATTAAGGTACAGCCTACTTGAAACAGGACACAACCTCAACTAACGGGTAATTTCAGTACA--CCTTATTTTCAAGTAGGCTTAAAAGCAGCCACCTTTCA-GATAGCGTCAAAGCTTTATTATAC-ATGTACTCAATACCTAATCTGTTATTTAACCCTTTATTAATATCGAGTAAATCTATAAAACCTATAGAAACTTTTATGTTAAAACTAGTAACAAGAACTAGACTCTCTC-TATATGTAAGTATACGTCAGAAAGGACCCTCCACTGACTATTAACACCCATGAACAAAA-AGAAGTAACTCAACAAGAAAACTCTTCTACACCAAGTGTTAATCTAACACAAGCACATATTAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACACCAACCCCGCCTGTTTACCAAAAACATCGCCTCTTGTA-AAATTTAAGAGGTCTAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATGAGGACTAGTATGAATGGCATCACGAGGGTTATACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTCCCCGTGAAGAAGCGGGGATCAATATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAACAACAACTGCTAATTTATTTAT-GACACTCAGGTTCCTAATCTTAACCCTAGCACTATGA-TCTTAAGTTTTTGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCACATTGAAAGTAAT--CCTTTCTAAGTAAAAAGTTACTACTTTAAACATCAACAAATTGACATTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCATTTTAAGAGCCCCTATCGACAAATGGGTTTACGACCTCGATGTTGGATCAGGGTATCCAAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAGT---------------------------------------------------------TACCTTATACCTCGTGTTTGGGGCATGAGCTGGGATAGTGGGGACCGCTCTTAGCCTCTTAATCCGAGCCGAGCTTAGCCAGCCCGGCTCGCTTCTGGGGGATGACCAAATTTATAACGTGATCGTCACTGCTCACGCCTTTGTAATAATCTTTTTTATAGTAATGCCGATTATAATCGGCGGATTTGGCAATTGACTGGTCCCATTAATAATTGGGGCCCCCGACATAGCCTTCCCTCGAATAAACAACATAAGTTTCTGACTCCTCCCTCCATCTTTTCTCCTCCTATTAGCCTCAGCTGGGGTCGAGGCAGGCGCCGGAACGGGGTGGACAGTATATCCGCCTCTTGCTAGCAACTTAGCACACGCCGGGCCATCTGTGGACCTCACTATTTTTTCTCTCCACTTGGCCGGGGTATCTTCAATCCTGGGGGCTATCAATTTTATTACTACAATTCTCAACATGAAGCCCCCCTCTGTAACCCAGTATCAAACCCCTCTTTTTGTTTGATCTGTTCTTATCACTGCCGTTCTCCTTCTTTTATCCCTTCCAGTTCTAGCTGCCGGAATTACTATGCTCCTCACAGACCGTAACCTAAACACAACATTCTTTGACCCAGCTGGGGGCGGGGATCCCATTCTATACCAACATTTATTC-------
Hylodes_pipilans --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAAAGCCTTTCGCTTACACCGAAGAACTATCTGTTAAACCCAGATTATTTTGAGCTAAAAATTTAA-CCCCACCTTCTTTTATGCCCACCACAAGACT-TAAATTATATAACTAAAACATTCTTACAGTTTAGTAGAGGCGATCAAAAAAC-TAACAGGAGTAATAAATAATGTACCGCAAGGGAAAAATGAAATAAAAATGAAATAC--TATCAAGCAAAAAAAAGTAAAGATTAATCCTTGTACCTTTTGCATCATGATTTAACTAGCCTACCCCGGCAAAACGACTTTTAAGTCAGACCTCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCTTATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGAGGTGACAGACCAAACGAACTAAGTGATAGCTGGTTACTCGAGAAAAGGATCTTAGTCCCGCCCAAAGAAAACACTATTA-ATTAAAAATAAAAATTTTCGACTTTAAAGCAATTCAATTAAGGTACAGCCTACTTGATACAGGAAACAACCTAAACTAACGGATAATCATTATACACCCTCCCCTTTTAAGTGGGCCTAAAAGCAGCCACCTTTAA-AATAGCGTCAAAGCTTCATTATAC-ATGTATCTAATACCAAATTTATTATTTAACCCTTTATTAATATCGAGTAAATCTATAAAA-CTATAGAAATTTTTATGTTAAAACTAGTAACAAGAGTTAGATTTTCTC-TTTATGTAAGTGTATATCAGAAAGGACACACCACTGACTATTAACATCTATGACCTTAG-AGCAGCAACTTGACAAGAAAACCCTCCTCCCCTAAATGTTAATCTAACACAAGCACATACCAAGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAACATTAACCCCGCCTGTTTACCAAAAACATCGCCTCTTGCA-AAACTTAAGAGGTCTAGCCTGCCCAGTGAC-TCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTACACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGGATCATTATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAACAACATTTGCTAACTCACCCGC-GATACTCAGGTCGCTAATCTTAATCCTAGCACTATGA-TTTCAAGTTTTTGGTTGGGGTGACCGCGGAGTAAAAATTAACCTCCACAATGAAAGAACT-----CTCTAAGTAAAAAGCTACTACTTTAAGCATCAATAAATTGACATTCATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTAAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTACCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAAC------------------------------------------------------------------------------------CTCTGTACCTTGTATTCGGGGCATGAGCCGGAATGGTGGGAACTGCCCTAAGCCTCCTGATTCGAGCTGAACTAAGTCAGCCCGGCTCACTATTAGGAGACGATCAGATTTATAATGTTATCGTCACCGCCCATGCCTTTGTAATAATTTTTTTTATAGTAATGCCTATTATAATCGGCGGATTCGGTAACTGACTGGTACCTCTAATAATCGGAGCCCCGGATATGGCCTTTCCCCGGATAAACAATATAAGCTTCTGACTCCTCCCTCCGTCTTTTCTTCTTCTATTAGCCTCAGCTGGTGTAGAAGCAGGCGCCGGGACGGGTTGAACAGTTTATCCCCCTCTTGCTAGCAACCTAGCCCATGCCGGACCCTCTGTAGACTTAACCATCTTTTCCCTTCACTTAGCTGGTGTGTCATCTATTCTTGGCGCTATTAATTTTATTACCACAATCATCAACATAAAACCTCCTTCAGTAACCCAATATCAAACCCCACTCTTCGTTTGATCTGTCCTCATTACCGCTGTCCTTCTACTCCTATCACTCCCAGTTCTGGCTGCCGGAATCACAATGCTCCTAACAGATCGAAACTTAAACACAACATTCTTTGACCCAGCTGGAGGAGGAGATCCCGTTCT-----------------------
Hylodes_sazimai -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGCTTAA-TTAAAGCCCTTCGCTTACACCGAAAAATTATCTGTTAGACCCAGATTATTTTGAGCTAAAAATCTAA-CCCCACCTTATATTATATCATCT-CAAGACT-TTAATCATTTAATTAAAACATTCTTATAGTTTAGTAGAGGCGATCAAAAAAT-TAATTGGAGTGATAAATACTGTACCGCAAGGGAAAGATGAAATAAAAATGAAAAAT--TATTAAGCACAAAAAAGTAAAGATTTACCCTTGTACCTTTTGCATCATGATTTAACTAGCTTACCCCAGCAAAACGA-CATTAAGTCGGACCCCCCGAAACTTAGTGAGCTACTTCAAGGCAGCTTAATGAGCACATCCGTCTCTGTTGCAAAAGAGTGGAGAGACCTTCAAGTAGCGGTGATAGACCTAACGAACTAAGTGATAGCTGGTTACTCAAGAAAAGAATTTTAGTTCCACCTAAAGCAAACATTATTA-CTTTTAAATAAGAATTTCTAGCTTTAGAGCAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTTGACTAATGGGTAACTATTATATA--CCTCATTTTTAAGTGGGCCTAAAAGCAGCCACCTTTAATAATAGCGTCAAAGCTTTATTATGC-AAATATTTAATACCAATTTTTTTTCATAACCCTTTATTAGTACTGAGTAAATCTATATAA-TTATAGAAACTTTTATGTTAAAACTAGTAACAAGAATTAGATTTTCTC-TTTATGCAGGTGTATATCAGAAAGGACATACCACTGATAATTACCATTCATGAACCTAA-AGAAGCAACTTAACTAGAAAACTCTTCTATAATCAATGTTAATCTAACACAAGAGCATATCANGAAAGATTAAAAGAAAAAGAAGGAACTCGGCAAATATTAGCCTCGCCTGTTTACCAAAAACATCGCCTCTTGC--AAATCTAAGAGGTCCAGCCTGCCCAGTGAC-TCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGCTTTACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATCGATTTATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAATAACACCTGCTAATCCACTTAT-AATATTCAGATACTTTATTTATATCTTAGCACTATGA-TTACAAGTTTTAGGTTGGGGTGACCGCGGAGTAAAAACCAACCTCCACATTGAAAGAACT---CTTTCTAAGCAAAAAGCTACAACTTTAAGCATCAATAAATTGACACTTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTTGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCT--------------------------------------------------------------------------------------------------------ACAGCTCTAAGCCTACTCATCCGAGCCGAACTAAGCCAGCCTGGCTCACTTTTAGGAGATGACCAAATTTATAATGTTATCGTTACCGCACATGCTTTTGTTATAATTTTTTTCATAGTAATGCCTATTATAATTGGTGGGTTCGGTAATTGACTGGTCCCCCTAATAATTGGAGCTCCAGATATGGCCTTTCCACGAATAAATAATATGAGTTTTTGACTCCTCCCTCCCTCCTTCCTTCTTCTTTTGGCCTCCGCCGGAGTTGAAGCAGGCGCAGGAACAGGGTGAACAGTCTACCCCCCTCTTGCCAGCAACTTAGCCCATGCTGGGCCCTCTGTAGATCTCACCATTTTTTCTCTTCACCTTGCCGGCGTATCGTCCATCCTTGGTGCCATTAATTTTATCACTACGATTCTAAACATAAAACCCCCATCCATAACCCAATATCAAACCCCCCTTTTTGTCTGATCTGTTTTAATCACTGCTGTTCTTCTTCTCTTATCCCTTCCGGTTTTAGCCGCTGGTATCACAATGCTTCTCACGGATCGAAATTTAAATACAACATTTTTTGACCCGGCAGGTGGAGGGGACCCCATTCTTTACCAACATTTATTTTGATTCT
Hylodes_uai -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGCCTGTTTATCAAAAACATCGCCTCTTGC--AAATTTAAGAGGTCTAGCCTGCCCAGTGAC-TTTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCATCACGAGGGTTACACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTACTATATAAGACGAGAAGACCCTATGGAGCTTTAAAC-AAGTAACATCTGCTAAT-TAATTAT-CATATTCAGATATCTAACTTTTAACCTAGCACTATTACCTACTAGTTTTTGGTTGGGGTGACCGCGGAGTACAAAATAACCTCCACATTGAAAGAACT---CTTTCTAAGCAAAAAACTACAATTTTAAGCATCAACAAATTGACATCTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTCAAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCTACGTGATCTGAGTTCAGACCGG-????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
Megaelosia_apuana ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????----------------------------------------------GAACCCTATACCTGGTTTTCGGGGCATGAGCCGGAATAGTCGGAACAGCCCTAAGTCTTCTAATCCGAGCCGAATTAAGCCAGCCCGGCTCCCTCCTTGGAGACGATCAAATTTATAACGTTATTGTTACCGCTCACGCTTTCGTCATGATTTTCTTCATGGTGATACCCATCATGATTGGAGGTTTTGGTAATTGACTAGTACCTCTTATAATTGGGGCCCCTGATATGGCCTTCCCTCGAATAAACAACATAAGTTTTTGACTTCTACCCCCGTCCTTTCTTCTACTCCTTGCCTCCGCCGGCGTTGAGGCGGGGGCCGGCACCGGGTGGACAGTCTACCCCCCTCTTGCCGGTAATTTAGCCCATGCCGGCCCCTCTGTAGATCTAACCATCTTTTCCCTCCACCTTGCCGGTGTGTCATCAATCCTTGGGGCCATTAACTTTATCACCACAATCCTTAATATAAAACCTCCCTCAATAACCCAATATCAGACGCCTCTTTTTGTCTGATCTGTTCTGATTACAGCTGTCCTTCTTCTTTTATCCTTGCCTGTCTTAGCCGCCGGAATCACAATACTGCTTACAGACCGTAACCTAAACACAACCTTCTTTGACCCGGCTGGGGGTGGAGATCCAGTTCTCTAT-------------------
Megaelosia_boticariana -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AGCTTAG-CTAAAGCACCTCGCTTACACCGAGTAAACATCTGTGAAACCCAGATTATTTTGAGCCAAAAATCTAG-CCCTATCTC-CCCCATGTAAACT-CAACATT-TCAAATTATAAATCAAAACATTTTAATGATTAAGTAAAGGCGATTGAAAAAT-TAACAGGAGCTATATACAATGTACCGCAAGGGAAAGATGAAATAAAAATGAAATATT-TATCAAGCACAAAAAAGTAAAGATTAATTCTTGTACCTTTTGCATCATGATTTAACTAGTCTACCCTAGCAAAACGA-TTTTTAGTTTGACCCCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCAAATCCGTCTCTGTTGCAAAAGAGTGGAAAGACCTTCAAGTAGGGGTGACAGACCTAACGAACTTAGTGATAGCTGGTTACTCAAGAAAAGGATCTAAGTCCAACCTAAAACTTAACTTATAC-CACAAGATTAAAAACTT--AACTTTAGAGCAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTCAACTAATGGGTTATCTTAACCAC--CAGCACATTTAAGTTGGCCTAAGAGCAGCCACCTTTTA-AATAGCGTCAAAGCTTAGCCATGC-GACTGTTTAATCTCAACAACACCTATTAACCCTTTACTACTATTGAGTACTTCTATATAC-CTATAGAACTTTTTATGTTAAAACTAGTAACAAGAACTAGGCCTTCTCTTATATGTCAGTGTATATCAGTAAGGACACACCACTGATAATTAACACCAATGAACTAAA-AGTAGCAACTCAACAAGAAAACCCTTCTTAATAATATGTTAATCTAACACAAGTACATTCCAAGAAAGATTTAAAGAAAAAGAAGGAACTCGGCAAATATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTAAAAATTTAAGAGGTCCAGCCTGCCCAGTGAC-CCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAAGGTTATACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATAATTATATAAGACGAGAAGACCCTATGGAGCTTTAAACAAAATAACAATTGCTAAC-TAAATCT-TAATTTCAGAAAACCAATCTTTATCCTAGCATTATGA-TTACCAGTTTTAGGTTGGGGTGACCACGGAGAAAAAAGTAACCTCCATATTGAAAGAACTA-CCATTCTAAGCAAAAAGCTACAACTTTAAGCATCAACAAATTGACATCTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCTATCCACTTTAAGAGCCCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCCAGTGGTGCAGCCGCTACTAAAGGTTCG-TTGTTCAACGATAAAACC--------------------------GTGATAATTACCCGATGATTTTTTTCAACTAACCATAAAGATATTGGAACCCTATACCTGGTCTTCGGGGCATGAGCCGGAATAGTCGGAACAGCCCTAAGTCTCCTAATCCGAGCCGAATTAAGCCAACCCGGCTCTCTCCTCGGAGATGACCAAATTTATAATGTTATTGTTACCGCCCACGCTTTCGTCATGATTTTTTTCATGGTAATACCCATCATAATTGGAGGCTTTGGTAACTGACTCGTACCCCTTATAATTGGAGCCCCTGATATAGCTTTCCCCCGAATGAACAACATAAGCTTCTGACTTCTACCACCCTCCTTTCTTCTACTTCTTGCCTCCGCCGGCGTTGAAGCAGGGGCCGGCACCGGGTGAACAGTTTATCCCCCTCTTGCCGGTAATATAGCCCACGCTGGCCCCTCTGTAGACCTAACTATTTTTTCTCTTCACCTTGCCGGTGTGTCATCAATTCTTGGGGCCATTAATTTTATTACTACAATCCTTAATATAAAACCCCCTTCAATAACCCAGTATCAAACGCCTCTTTTTGTCTGATCTGTCTTGATCACAGCTGTCCTTCTTCTCCTGTCCCTACCCGTTTTAGCCGCCGGAATTACAATACTTCTCACAGACCGAAATGTAAACACAACCTTTTTTGACCCAGCTGG-----------------------------------------
Megaelosia_goeldii GTTTGGTCCTAACCTTGTAATCAATTTTTACTTAATATACACATGCAAGTCTCCGCACCCCTGTGAAAACGCCCTTAAATCCCCCAT--GGGATAA-GGAGCTGGTATCAGGCACGAAAATCTGCCCAAAACACCTAGCTATGCCACACCCACAAGGGTACTCAGCAGTGATTGACATTATTTATAAGCGCCAGCTTGACTCAGTTAAAGTAAAGAGAGCCGGCAAATCTGGTGCCAGCCGCCGCGGTTACCCCACGTGGCTCAAATTGATTTCTTTCGGCGTAAAGCGTGATTAAAGTGCCCATCAACATTGGAGTTAAACTAAAATTAAGCTGTGACACGCTTATTTTACAGAAAAGCACAAACGAAAGTTACTTCAATTTAAACAACTTGAATTCACGACAGTCAGGACACAAACTGGGATTAGATACCCCACTATGCCCGACCGTAAACTTTAATTTACACCAT-CACCGCCAGAGAACTACGAGCAAAGCTTAAAACTCAAAGGACTTGACGGTCCCCCACATCCCCCTAGAGGAGCCTGTCCTTTAATCGATAATCCCCGCTTAACCTCACCATTCTTAGTCTTTCAGCCTGTATACCTCCGTCGTCAGCTTACCCCGTGAGCGAAAATTAGTGAGCTTAATGTCCACACGTCTACACGTCAGGTCAAGGTGCAGCAAATATAATGGGAAGAGATGGGCTACACTTTCTAGTCTAGAATATACGAAAGACCACCTATGAAA-CCTGGTCAGAAGGCGGATTTAGAAGTAAAAGGAAACCAGAGCATCCCTTTTAATTTGGCACTGGGGCATGTACACACCGCCCGTCACCCTCTTCA-AAGCCTAATTTTAGTATCTAACCAACTAACG-CCTAGTAGAAGAGGCAAGTCGTAACATGGTAAGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCCTAA-TCAAAGCATTTCGCTTACACCGAAAAGTTATCTGTGAAATTCAGATTATTTTGAGCTAAAAATCTAG-CCCCACTTTATTCTATAATCCCT-TATCACT-TAAATTCATGAATCAAAACATTTTAATAATCAAGTAAAGGCGATTGAAAAAT-TAATAGGAGCAATATATACTGTACCGCAAGGGAAAGATGAAATAGAAATGAAATAATAATTAAAGCATAAAAAAGTAAAGATTAAATCTTGTACCTTTTGCATCATGATTTAACTAGTCTACCCAGGCAAAATGA-TTTTAAGTCTGACCTCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCAAATCCGTCTCTGTCGCAAAAGAGTGGAGAGACCTTCAAGTAGAAGTGATAGACCTAACGAACTTAGTAATAGCTGGTTATTCAAGAAAAGGATCTCAGTCCAACCTAAAGTCAAAT-TAATG-TTTAAAAATAAAAATTC-TGACCTTAGAGTAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTTAACTAATGGGTAAC--TTACCCC--TTCATCTTTTAAGTGGGCCTAAAAGCAGCCACCTTTAA-AATAGCGTCAAAGCTTAGCCGTCCTATACATCTAATACCAAAAACATCTATGAACCCTATACTCATATTGAATAATTCTATATTA-TTATAGAGATTTTTATGTTAAAACTAGTAACAAGAATTAAATTTTCTCTATTATGTTCGTGTACATCAGAAAGGATAAACCACTGATAATTGACATGCATGAGTAAAA-AGCAGTAACTTAACAAGAAAACCCTCCTAACTCTAATGTTAACCTAACACAAGTACATCTCAAGAAAGATTTAAAGAAAAAGAAGGAACTCGGCAAACATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTCAAAATTTAAGAGGTCCAGCCTGCCCAGTGAC-CCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAGGGTTATACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATTTTTATATAAGACGAGAAGACCCTATGGAGCTTTAGAC-GAGTAACAACTGCTAAT-TTTATAA-TAT-TTCAGATAAT--ATCTCTATCCTAGCATTATGA-TTATAAGTCTTTGGTTGGGGTGACCGCGGAGAAAAAAATAACCTCCACATTGAAAGAATA--TTATTCTAAGCAAAAAGACACATCTTTAAGCATCAACAAATTGACATCTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCAATCCACTTCGAGAGCTCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTATCCTAGTGGTGTAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT----------------------------------------------------------------------AACCTTATACCTTGTATTTGGGGCATGAGCTGGTATAGTGGGCACCGCCCTAAGCCTCCTTATTCGAGCTGAATTAAGCCAACCCGGCTCTTTACTCGGAGACGACCAAATTTATAACGTCATTGTTACCGCCCACGCCTTTGTTATAATCTTCTTCATAGTAATACCAATCATGATTGGAGGTTTCGGTAACTGACTTGTGCCACTAATAATTGGAGCCCCCGACATAGCTTTCCCTCGAATAAATAATATAAGCTTTTGACTCCTGCCCCCATCTTTTCTTCTTCTTCTCGCCTCTGCCGGCGTTGAAGCGGGCGCTGGCACAGGCTGGACAGTATACCCGCCACTTGCGGGAAACCTAGCTCACGCCGGCCCCTCTGTTGACCTTACCATTTTTTCTCTTCATCTCGCCGGTGTTTCATCTATTCTTGGAGCCATTAATTTTATTACGACAATCCTTAATATAAAACCCCCATCAATAACCCAATACCAAACACCTCTTTTCGTCTGATCCGTCCTTATCACTGCCGTTCTCCTTCTCTTATCTCTTCCTGTCTTAGCAGCAGGCATCACCATGCTTCTTACAGACCGCAACTTAAATACAACATTCTTTGATCCTGCAGGAGGGGGGGACCCAGTTCTTTATCAACATCTGTTT-------
Megaelosia_jordanensis GTTTGGTCCTAACCTTGTAATCAATTCTTACTTAATATACACATGCAAGTCTCCGCCTCCCTGTGAAAACGCCCTTAAA-TCCCCATTAGGGATAA-GGAGCCGGTATCAGGCACGATAATCTGCCCAAAACACCTAGCTATGCCACACCCACAAGGG-ACTCAGCAGTGATTAACATTGAACATAAGCGTCAGCTTGACTCAGTTAAAGTAAAGAGAGCCGGCAAATCTGGTGCCAGCCGCCGCGGTTACCCCACGTGGCTCAAATTGATTTCTATCGGCGTAAAGCGTGATTAAAGTCCCCATT-ATATTGAAGTTAAACTGAAACTAAGCTGTGACACGCTTGTTACACAGAAAACCATAAACGAAAGTTACTTCAACTTT-TCAACTTGAATACACGACAGCTAGGGCACAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTAATTACACCCCCCAACGCCCGAGAACTACGAGCAAAGCTTAAAACTCAAAGGACTTGACGGTACCCCATATCCCCCTAGAGGAGCCTGTCCTATAATCGATAATCCCCGTTTAACCTCACCACTTTTAGTTTTTCAGCCTGTATACCTCCGTCGTCAGCTTACCACGTGAGCGAAATTTAGTGAGCTTAATGTCCACACGCCCACACGTCAGGTCAAGGTGCAGCAAATGAAGTGGGAAGAGATGGGCTACACTTTCTATATTAGAAAACACGAAAGACCACACATGAAA-CTTGGTCAGAAGGCGGATTTAGTAGTAAAAAGAAATCAAAATGTTCTTTTTAATTCGGCACTGGGGTGTGTACACACCGCCCGTCACCCTCTTCATAAGCCTAACTCTAGTATTTAACCAACTTATGCCCGAATAGAAGAGGCAAGTCGTAACATGGTAAGTATACCGGAAGGTGTGCTTGGAAACAAAATATAGCTTAA-CTAAAGCACCTCGCTTACACCGAGTAAACATCTGTGAGACCCAGATTATTTTGAGCCAAAAATCTAG-CCCCTCTCC-CCCCATGTAAACT-CAACATT-TCAGATTATAAACTAAAACATTTTAATAATAGAGTAAAGGCGATTGAAAAAT-TAACAGGAGCTATATATAATGTACCGCAAGGGAAAGATGAAATAAAAATGAAATATT-TATTAAGCACTAAAAAGTAAAGATTAACTCTTGTACCTTTTGCATCATGATTTAACTAGTTTACCCTAGCAAAACGA-TTTTTAGTTTGACTTCCCGAAACTAAGTGAGCTACTTCAAGGCAGCTTAATGAGCAAATCCGTCTCTGTTGCAAAAGAGTGGAAAGACCTTCAAGTAGGGGTGACAGACCTAACGAGCTTAGTGATAGCTGGTTACTCAAGAAAAGGATCTAAGTCCAACCTAAAGCTTAATTTAACA-CCACAAATTAAAAACTT--AACTTTAGAGCAATTCAATTAAGGTACAGCCTACTTGAAACAGGATACAACCTCAACTAATGGGTTATTTTAACCAT--CAACACATTTAAGTTGGCCTAAGAGCAGCCACCTTTTA-AATAGCGTCAAAGCTTAGCCATGC-AACTGTTTAATCTCAACAACACCTTTTAACCCTTTACTACTATTGAGTACTTCTATATAC-CTATAGAACTTTTTATGTTAAAACTAGTAACAAGAACCAGACCTTCTCTTATATGTCAGTGTACATCAGTAAGGACACACCACTGATAATTAACACCAATGAACTAAA-AGTAGCAACTCAGCAAGAAAACTCTTCTTAACAATATGTTAATCTAACACAAGTACATTCCAAGAAAGATTTAAAGAAAAAGAAGGAACTCGGCAAACATTAACCTCGCCTGTTTACCAAAAACATCGCCTCTTGTAGAAATTTAAGAGGTCCAGCCTGCCCAGTGAC-TCTGTTCAACGGCCGCGGTATCCTAACCGTGCGAAGGTAGCGTAATCACTTGTTCTTTAAATAAGGACTAGTATGAATGGCACCACGAAGGTTACACTGTCTCCTTTTTCTAATCAGTGAAACTAATCTTCCCGTGAAGAAGCGGGAATAATTATATAAGACGAGAAGACCCTATGGAGCTTTAAACAAAGTAACAACTGCTAAC-TAAATCT-TAATTTCAGAAAACCAATCTTTAACCTAGCATTGTGA-TTACTAGTTTTAGGTTGGGGTGACCGCGGAGCAAAAAACAACCTCCATATTGAAAGAACTA-TAATTCTAAGCAAAAAGCTACAACTTTAAGCATCAACAAATTGACATCTATTGACCCAATA--TTTTGATCAACGAA-CCAAGTTACCCTAGGGATAAC--AGCGCTATCCACTTTAAGAGCCCTTATCGACAAGTGGGCTTACGACCTCGATGTTGGATCAGGGTACCCCGGTGGTGCAGCCGCTACTAAAGGTTCGTTTGTTCAACGATTAAAACCCT-----------------------????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????
;
END;
Uma das vantagens do formato nexus é que é um formato em blocos. Este acima é o bloco que contem sua matriz (é chamado data block), e ele começa em BEGIN DATA; e termina em END;. Então, ao invés de escrevermos todos os comandos no console do MrBayes, os escreveremos num próximo bloco.
Uso local
Vamos preparar nosso bloco com os comandos do MrBayes. Num editor de texto, adicione o seguinte no final do arquivo hylodidae.nex:
begin mrbayes;
charset h1 = 1-2475;
charset coi_pos1 = 2476-3187\3;
charset coi_pos2 = 2477-3187\3;
charset coi_pos3 = 2478-3187\3;
partition PartitionFinder = 4:h1, coi_pos1, coi_pos2, coi_pos3;
set partition=PartitionFinder;
lset applyto=(1) nst=6 rates=invgamma;
lset applyto=(2) nst=6 rates=propinv;
lset applyto=(3) nst=1 rates=propinv;
lset applyto=(4) nst=6 rates=gamma;
prset applyto=(all) ratepr=variable;
unlink statefreq=(all) revmat=(all) shape=(all) pinvar=(all) tratio=(all);
Ao invés de explicarmos cada um desses parâmetros aqui, porque não abrimos o MrBayes e deixamos ele nos dizer o que cada um significa? Digite o comando help seguido de um outro comando, como por exemplo:
MrBayes > help charset
---------------------------------------------------------------------------
Charset
This command defines a character set. The format for the charset command
is
charset <name> = <character numbers>
For example, "charset first_pos = 1-720\3" defines a character set
called "first_pos" that includes every third site from 1 to 720.
The character set name cannot have any spaces in it. The slash (\)
is a nifty way of telling the program to assign every third (or
second, or fifth, or whatever) character to the character set.
This option is best used not from the command line, but rather as a
line in the mrbayes block of a file. Note that you can use "." to
stand in for the last character (e.g., charset 1-.\3).
---------------------------------------------------------------------------
O programa está nos dizendo que o comando charset define uma partição. Agora faça com os outros comandos.
Vamos adicionar o resto dos comandos e fechar o bloco:
mcmc ngen=100000 nruns=2 nchains=4 samplefreq=1000 printfreq=1000 relburnin=yes burninfrac=0.25;
sump;
sumt;
end;
Isso indica que vamos rodar duas corridas com quatro correntes cada uma de 100.000 gerações, amostrando e printando na tela a cada 1.000 gerações, descartando os primeiros 25% das amostras como burnin. Este descarte ocorre porque no começo a busca pode estar em um lugar muito distante no espaço amostral da nossa resposta ótima, o que pode atrapalhar nosso resultado final. Então é melhor esperar as corridas se estabilizarem para começar a considerar os resultados.
Agora salve este novo arquivo como hylodes_local.nex e coloque-o no mesmo diretório onde está instalado o MrBayes. Abra o console e escreva:
exe hylodidae_local.nex
Aperte a tecla enter e espere a análise rodar, deve demorar alguns poucos minutos. Ao final da sua análise, a seguinte mensagem irá parecer:
Continue with analysis? (yes/no):
Escreva no e aperte enter. Pronto! Agora temos que checar se nossas corridas convergiram, ou seja, se deu tudo certo com a nossa análise. Primeiro, abra o arquivo log.out. Agora, temos que checar três fatores para ter certeza que isso aconteceu:
1) Average standard deviation of split frequencies:
Deve ser menor que 0.01, você o encontra logo depois que suas corridas terminam:
96000 -- (-14945.919) (-14946.775) [-14941.347] (-14940.774) * (-14941.137) (-14938.274) [-14925.970] (-14960.757) -- 0:00:09
97000 -- [-14934.166] (-14940.641) (-14929.078) (-14953.398) * (-14935.327) (-14958.063) [-14936.247] (-14966.281) -- 0:00:07
98000 -- (-14951.948) (-14963.225) [-14935.903] (-14934.229) * (-14941.401) [-14932.658] (-14931.962) (-14973.433) -- 0:00:04
99000 -- [-14937.668] (-14943.012) (-14930.462) (-14942.044) * (-14948.446) (-14926.865) (-14948.461) [-14944.319] -- 0:00:02
100000 -- [-14943.526] (-14934.642) (-14937.203) (-14946.220) * (-14960.036) (-14934.113) (-14941.928) [-14934.390] -- 0:00:00
Average standard deviation of split frequencies: 0.048329
2) ESS = Estimated Sample Size
É bom que seja maior que 100. Se for menor que 100, pode ser que o parâmetro em questão tenha sido subamostrado. Se encontra na mesma tabela que o próximo parâmetro.
3) PSRF = Potential Scale Reduction Factor; (Gelman and Rubin, 1992)
Deve estar próximo de 1.0 para considerarmos que as corridas convergiram.
95% HPD Interval
--------------------
Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+
----------------------------------------------------------------------------------------------------
TL{all} 3.313495 0.095620 2.752994 3.819549 3.298827 29.95 34.16 1.034
r(A<->C){1} 0.055433 0.000105 0.037193 0.073771 0.055014 36.94 42.79 1.028
r(A<->G){1} 0.164844 0.000244 0.135861 0.194041 0.163742 56.97 59.96 0.993
r(A<->T){1} 0.127138 0.000122 0.105205 0.146239 0.126518 44.18 60.09 1.002
r(C<->G){1} 0.009409 0.000019 0.002623 0.017466 0.008423 34.27 43.73 1.053
r(C<->T){1} 0.637683 0.000521 0.594351 0.680154 0.638765 30.05 42.62 0.994
r(G<->T){1} 0.005492 0.000009 0.000259 0.011026 0.005240 76.00 76.00 1.005
r(A<->C){2} 0.012578 0.000156 0.000047 0.036728 0.008242 22.14 29.40 1.001
r(A<->G){2} 0.082150 0.001852 0.019381 0.165416 0.075450 42.95 56.70 0.997
r(A<->T){2} 0.040709 0.000797 0.000346 0.090616 0.033522 15.83 26.98 1.025
r(C<->G){2} 0.007342 0.000043 0.000048 0.019648 0.005736 59.54 59.54 0.996
r(C<->T){2} 0.843747 0.003770 0.722904 0.936531 0.851874 37.76 47.13 0.997
r(G<->T){2} 0.013473 0.000165 0.000001 0.035842 0.009640 32.89 54.44 0.996
r(A<->C){4} 0.020963 0.000074 0.003998 0.036994 0.021144 46.92 47.74 1.018
r(A<->G){4} 0.607019 0.003032 0.517721 0.709761 0.602735 14.51 30.27 1.000
r(A<->T){4} 0.024717 0.000123 0.007256 0.045945 0.023977 23.90 29.55 1.030
r(C<->G){4} 0.025374 0.000274 0.000220 0.054081 0.025028 15.14 17.49 0.993
r(C<->T){4} 0.290922 0.002154 0.200477 0.372007 0.291767 19.42 45.67 0.997
r(G<->T){4} 0.031005 0.000276 0.002377 0.062168 0.031621 20.36 26.85 1.007
pi(A){1} 0.338609 0.000066 0.321661 0.352347 0.338749 20.30 48.15 0.997
pi(C){1} 0.204615 0.000047 0.192655 0.217897 0.203805 31.01 37.96 0.994
pi(G){1} 0.174911 0.000061 0.158678 0.188289 0.175326 40.62 55.04 1.012
pi(T){1} 0.281865 0.000055 0.266817 0.293445 0.283069 17.70 44.50 0.998
pi(A){2} 0.256994 0.000843 0.205362 0.313671 0.256906 49.91 62.95 0.997
pi(C){2} 0.291013 0.000587 0.239591 0.335195 0.291750 63.84 67.57 1.029
pi(G){2} 0.285427 0.000813 0.234344 0.336384 0.283324 54.23 65.12 1.019
pi(T){2} 0.166567 0.000371 0.133719 0.202801 0.166095 63.74 69.87 1.002
pi(A){3} 0.156525 0.000579 0.105161 0.196909 0.156786 41.58 45.19 1.008
pi(C){3} 0.276278 0.000782 0.225311 0.327313 0.275678 49.09 51.36 1.002
pi(G){3} 0.152130 0.000600 0.105573 0.196578 0.152691 57.64 62.39 0.995
pi(T){3} 0.415068 0.001069 0.355647 0.471419 0.414233 34.65 45.62 0.995
pi(A){4} 0.268792 0.000305 0.235415 0.301895 0.269083 39.98 51.50 1.000
pi(C){4} 0.309260 0.000229 0.279034 0.330491 0.308440 54.29 65.15 0.997
pi(G){4} 0.102812 0.000067 0.090115 0.119845 0.102732 16.38 46.19 0.993
pi(T){4} 0.319136 0.000189 0.293660 0.345194 0.319001 25.74 50.87 1.021
alpha{1} 0.674343 0.022632 0.444879 0.939185 0.662354 37.62 42.44 0.995
alpha{4} 1.811033 0.141191 1.247278 2.625227 1.740927 55.27 65.64 1.046
pinvar{1} 0.448232 0.002622 0.344596 0.522998 0.455662 37.66 40.24 0.999
pinvar{2} 0.777208 0.001983 0.689191 0.853191 0.779830 64.19 64.22 0.998
pinvar{3} 0.428470 0.081716 0.058842 0.999549 0.370237 15.57 26.94 1.000
m{1} 0.604601 0.002546 0.520221 0.690614 0.604241 27.35 37.18 1.020
m{2} 0.235658 0.002833 0.133341 0.331364 0.223827 46.59 55.66 1.005
m{3} 0.053227 0.051856 0.000013 0.330918 0.001268 76.00 76.00 0.994
m{4} 6.843503 0.300651 5.943427 7.852737 6.855526 31.79 45.85 1.026
----------------------------------------------------------------------------------------------------
E aí, suas corridas convergiram? Se tiver sido como a nossa análise, parece que não. O melhor a se fazer em casos assim é aumentar o número de gerações. Geralmente, algo em torno de 10 milhões é bem razoável para a maioria das análises. Porém, isso pode demorar demais! Vamos fazer mais uma análise, mas agora em um servidor.
Uso em servidor de alta performance (plataforma CIPRES)
No nosso módulo sobre verossimilhança nós já explicamos sobre o bom CIPRES Science Gateway. Há também uma versão do MrBayes instalada no portal, e vamos utilizá-la para nossa análise.
Abra o arquivo hylodidae_local.nex e mude o número de gerações de 100.000 para 1.000.000 e salve o novo arquivo como hylodidae_cipres.nex. Ele vai ficar assim:
mcmc ngen=1000000 nruns=2 nchains=4 samplefreq=1000 printfreq=1000 relburnin=yes burninfrac=0.25;
Agora vá até o CIPRES Science Gateway e siga os passos:
1) Após logar novamente na sua conta, vá até a pasta que você criou anteriormente, clique em Data e então em Upload Data. Depois clique novamente em Escolher arquivos, faça o upload do arquivo hylodidae_cipres.nex e clique em save.
2) Agora clique em Tasks e então em Create New Task. Vá em Select Input Data e selecione hylodidae_cipres.nex, depois clique em select data.
3) Vá em Select Tool e seleciuone a opção MrBayes on XSEDE (3.2.6). Depois clique em 148 parameters set.
4) Como você fez um Data Block do MrBayes, toda a informação da sua análise está lá. Então clique em My Data Contains a MrBayes Data Block (CHECK THIS OR MrBayes BLOCK ENTRIES WILL BE OVERWRITTEN!!!). Clique em Save Parameters e depois em ok.
5) Uma caixa de avisos avisando que o MrBayes utilizado juntamente com o BEAGLE pode ocasionar topologias estranhas. Clique em ok. O BEAGLE é um programa que ajuda muito na velocidade das análises, visto que otimiza o uso dos processadores.
6) Clique em Save and Run Task, depois em OK e espere a análise terminar. Você pode acompanhar o andamento da sua análise clicando em View Status, depois em Intermediate Results e baixando o arquivo stdout.txt.
Agora é só esperar alguns minutos até sua análise terminar.
Depois da análise terminada, baixe os arquivos stdout.txt e infile.nex.con.tre. Abra o primeiro arquivo em um editor de texto e analise a convergência das corridas. Com 10 vezes mais gerações, os parâmetros melhoraram? Dessa vez todos os parâmetros foram amostrados suficientemente?
Abra o arquivo infile.nex.con.tre com o FigTree. Note que há algumas politomias. Isso ocorre porque esta árvore é uma árvore de consenso de maioria, onde clados com menos de 0.5 (ou 50%) de probabilidade posterior são colapsados.
É isso, pessoal! Espero que tenham gostado. Qualquer coisa estamos á disposição!
Obrigado!!!
Referências
Gelman A, Rubin DB. (1992). Inference from iterative simulation using multiple sequences. Statistical Sciences 7:457–472.
Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck, JP. (2012). MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61:539-542.